PMID: 9553798Apr 29, 1998Paper

Rapid cooling, moderate heat treatment and nisin addition influence cell homeostasis of Clostridium perfringens type A

International Journal of Food Microbiology
P GuerlavaJ L Tholozan

Abstract

Vegetative cells of Clostridium perfringens type A were exposed to heat shock (50 degrees C), cold shock (4 degrees C) and nisin, respectively. In all cases, pronounced leaks of cellular K+ were observed. These losses were not systematically related to cell death, as demonstrated by cell viability measurement by the most probably number method. During heat shock treatment, a 90% decrease of initial population was measured within 15 min, and K+ leaks were mainly associated to cell death. A cold shock induced a rapid and large K+ efflux from the cells, at least 50% of the total potassium content. Potassium movements were not associated with internal pH changes, or with cell death during cooling at 4 degrees C. After nisin addition up to 70% of the cellular potassium was lost within 5 min. These potassium losses led to a transmembrane pH gradient decrease, but not to cell death at 50 IU ml-1 of nisin. Increasing the nisin concentration to 200 IU ml-1 resulted in a decimal reduction of 2.62.

References

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Jan 1, 1996·Letters in Applied Microbiology·E HuotH Petitdemange
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Citations

Feb 23, 2011·Applied Biochemistry and Biotechnology·Yulong GaoQiang Fu
Dec 3, 2011·Journal of Food Science·Pathima UdompijitkulMahfuzur R Sarker
Jun 25, 2013·Comparative Immunology, Microbiology and Infectious Diseases·Janneke G AllaartAndrea Gröne
Jan 26, 2017·Médecine sciences : M/S·Bernard MartelNemeshwaree Behary Massika
Apr 21, 2018·International Journal of Microbiology·Miseon Park, Fatemeh Rafii
Jul 14, 2018·Scientific Reports·Vaiva KazanavičiūtėAušra Ražanskienė
Mar 2, 1999·Applied and Environmental Microbiology·J L TholozanM Federighi

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