Rapid creation system of morphologically and functionally communicative three-dimensional cell-dense tissue by centrifugation

Biotechnology Progress
Yuji HaraguchiTatsuya Shimizu

Abstract

This study reports a rapid fabrication system of a morphologically and functionally communicative three-dimensional (3D) cell-dense tissue without scaffolds by centrifugation. The tight adhesion between C2C12 myoblasts and culture surface was accelerated without significant cell damage by centrifugation (80 x g, 37 °C, 30 min). A thicker tissue created on a temperature-responsive culture surface was harvested by decreasing temperature. The 3D myoblast tissues having approximately 200 μm-thickness were created at 1.5 h [centrifugation (80 x g, 37 °C) for 30 min and tissue harvest for 1 h]. However, in the case of without centrifugation, the myoblast tissues had fragile parts even at 7.5 h after the incubation. Additionally, electrically/functionally communicative and thicker human induced pluripotent stem (iPS) cell-derived cardiac tissues were created rapidly by the centrifugation and cultivation at 37 °C. We report a centrifugation system that significantly shortens the creation time of 3D tissues. We envision that this procedure will contribute to the field of tissue engineering and regenerative medicine. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:1447-1453, 2018.

References

Apr 4, 2001·Artificial Organs·K FunatsuK Sugimachi
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·D C ColterD J Prockop
Apr 18, 2003·Biomaterials·Tatsuya ShimizuTeruo Okano
Sep 17, 2004·The New England Journal of Medicine·Kohji NishidaYasuo Tano
Apr 27, 2005·Circulation·Michael HofmannHelmut Drexler
Apr 10, 2009·Current Molecular Medicine·Jia LiuPieter A Doevendans
Aug 14, 2009·Expert Review of Cardiovascular Therapy·Juan C Chachques
Dec 17, 2009·Journal of Tissue Engineering and Regenerative Medicine·Yuji HaraguchiTeruo Okano
Feb 16, 2010·Advanced Drug Delivery Reviews·Jens M Kelm, Martin Fussenegger
Jun 30, 2011·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Kouhei NiyamaMutsumi Takagi
Nov 19, 2011·Progress in Molecular Biology and Translational Science·Akira Ito, Masamichi Kamihira
Jul 31, 2012·Biochemical and Biophysical Research Communications·Katsuhisa MatsuuraTeruo Okano
Jun 4, 2013·Journal of Tissue Engineering and Regenerative Medicine·Yuji HaraguchiTeruo Okano
Jul 25, 2013·Journal of Tissue Engineering and Regenerative Medicine·Takanori IwataTeruo Okano
Dec 3, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Masato SatoJoji Mochida
Jul 30, 2014·Methods in Molecular Biology·Yuji HaraguchiTeruo Okano
Apr 8, 2015·Journal of Tissue Engineering and Regenerative Medicine·Takanori HamaHiromi Kojima
Apr 29, 2015·Circulation Journal : Official Journal of the Japanese Circulation Society·Yoshiki SawaShigeru Miyagawa
Jun 23, 2015·Journal of Biomedical Materials Research. Part a·Akiyuki HasegawaTeruo Okano
Feb 19, 2016·Scientific Reports·Katsuhisa MatsuuraTeruo Okano

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Citations

May 31, 2019·Biotechnology Progress·Yuji HaraguchiTatsuya Shimizu

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