Rapid cryopreservation of sheep embryos by direct transfer into liquid nitrogen vapour at -180 degrees C

Reproduction, Fertility, and Development
A SzéllR Hudson

Abstract

The in vitro survival of control and rapidly cryopreserved sheep embryos was examined as a function of the duration of exposure to a vitrification medium (25% glycerol + 25% propylene glycol). Embryos in late morula to late blastocyst stages were permeated by a mixture of 10% glycerol + 20% propylene glycol for 10 min at 18-23 degrees C and then exposed to the vitrification medium for 0.5, 1, 2 or 4 min at 18-23 or 4-12 degrees C. The cryoprotectants were removed without cryopreservation (control embryos) or after rapid cryopreservation by direct transfer into liquid nitrogen vapour at -180 degrees C. The duration of exposure to the vitrification medium at 18-23 degrees C affected the in vitro survival rate of control embryos (P = 0.06) but had no effect on the survival of rapidly cryopreserved embryos. However, at 4-12 degrees C the duration of exposure affected the survival of cryopreserved embryos (0.5 min: 64%, 18/28; 1-4 min: 43%, 34/80; P = 0.074). Overall, the in vitro survival rate of control and cryopreserved embryos increased with advancing development from late morulae (36%) to late blastocysts (70%). The in vivo survival of embryos that had been exposed to the vitrification medium for 0.5 min at 4-12 degrees C and t...Continue Reading

Citations

Sep 1, 1991·Theriogenology·A Széll, R H Hudson
Oct 1, 1994·Theriogenology·A Z Széll, D P Windsor
Jan 1, 1994·Biotechnology Advances·Z Smorag, B Gajda
Aug 2, 2001·Theriogenology·G BarilP Mermillod
Mar 25, 2000·Theriogenology·A G Martínez, M Matkovic
Mar 3, 2012·Animal Reproduction Science·G S Amiridis, S Cseh
May 1, 2001·Reproduction in Domestic Animals = Zuchthygiene·A Massip
Jan 26, 2002·Animal Biotechnology·S E ZhuY F Chen

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