Rapid, culture-independent, optical diagnostics of centrifugally captured bacteria from urine samples

Biomicrofluidics
Ulrich-Christian SchröderUte Neugebauer

Abstract

This work presents a polymeric centrifugal microfluidic platform for the rapid and sensitive identification of bacteria directly from urine, thus eliminating time-consuming cultivation steps. This "Lab-on-a-Disc" platform utilizes the rotationally induced centrifugal field to efficiently capture bacteria directly from suspension within a glass-polymer hybrid chip. Once trapped in an array of small V-shaped structures, the bacteria are readily available for spectroscopic characterization, such as Raman spectroscopic fingerprinting, providing valuable information on the characteristics of the captured bacteria. Utilising fluorescence microscopy, quantification of the bacterial load has been achieved for concentrations above 2 × 10(-7) cells ml(-1) within a 4 μl sample. As a pilot application, we characterize urine samples from patients with urinary tract infections. Following minimal sample preparation, Raman spectra of the bacteria are recorded following centrifugal capture in stopped-flow sedimentation mode. Utilizing advanced analysis algorithms, including extended multiplicative scattering correction, high-quality Raman spectra of different pathogens, such as Escherichia coli or Enterococcus faecalis, are obtained from the an...Continue Reading

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Citations

Apr 5, 2016·Biochemistry Research International·Suprava PatelEli Mohapatra
Nov 28, 2015·International Journal of Antimicrobial Agents·Ute NeugebauerJürgen Popp
Dec 14, 2016·Analytical Chemistry·Samiksha NayakSamuel K Sia
Feb 6, 2016·Micromachines·Minghui TangHo-Pui Ho
Jan 29, 2016·Micromachines·Suzanne SmithJan G Korvink
Sep 29, 2018·Applied Spectroscopy·Susanne PahlowRohit Bhargava
Nov 15, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Andrea LockeAnita Mahadevan-Jansen
Aug 27, 2021·Advanced Drug Delivery Reviews·Benjamin A KuzmaConor L Evans

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