PMID: 8938521Oct 1, 1996Paper

Rapid debulking and CD34 enrichment of filgrastim-mobilized peripheral blood stem cells by semiautomated density gradient centrifugation in a closed system

Journal of Hematotherapy
D PrzepiorkaM Körbling

Abstract

Filgrastim-mobilized peripheral blood progenitor cells (PBPC) are used for hematopoietic reconstitution after myeloablative therapy for malignancies, but the large number of cells collected in a single apheresis frequently presents a problem for storage or further processing. We have evaluated the use of CD34 Buoyant Density Solution-PBPC, an ultralight-density colloidal silica suspension, for debulking and enrichment of CD34+ cells in PBPC preparations in a semiautomated system. Cells were collected from four filgrastim-treated normal donors using the COBE Spectra. The separation procedure was carried out with Plasma-Lyte A and DNase 5 U/ml using the COBE 2991. Following processing and washing, there was a 26% recovery of nucleated cells, 2.6-fold enrichment of CD34+ cells, 68% recovery of CD34+ cells, 88% recovery of CFU-GM, 73% recovery of BFU-E, 1 log depletion of CD3+ cells, 0.5 log depletion of CD56+ cells, and 1 log depletion of CD19+ cells. These results were not significantly different from those obtained when PBPC were separated over CD34 Buoyant Density Solution-PBPC by centrifugation in tubes. Using CD34 Buoyant Density Solution-PBPC, mononuclear preparations of PBPC can be enriched rapidly for CD34+ cells and deple...Continue Reading

References

May 1, 1994·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·R S Negrin, J Pesando
May 1, 1991·World Journal of Microbiology & Biotechnology·D C Smith, T M Wood

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Citations

Jun 21, 2001·In Vitro Cellular & Developmental Biology. Animal·C A SavaryJ H Rex
Apr 25, 2001·Bone Marrow Transplantation·M GrazziuttiC A Savary

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