Rapid decrease in transepithelial electrical resistance of human intestinal Caco-2 cell monolayers by cytotoxic membrane perturbents

Toxicology in Vitro : an International Journal Published in Association with BIBRA
A NaraiM Shimizu

Abstract

The transepithelial electrical resistance (TEER) of human intestinal Caco-2 cell monolayers cultured on a permeable filter rapidly decreased on apical exposure of the monolayer cells to such cytotoxic membrane perturbents as benzalkonium chloride and saponin. This decrease in TEER occurred at lower concentrations of these toxicants and at earlier stages of incubation than those for the release of intracellular lactate dehydrogenase, which is commonly used as a parameter for assessing cell membrane damage. The TEER decrease caused by the toxicants was associated with increased transepithelial permeability of the cell monolayer, as well as with subtle changes in the cytoskeletal structure, suggesting that membrane perturbation by the toxicants induced cytoskeletal changes that resulted in disruption of the tight junction and a subsequent increase in paracellular permeability. The rapid TEER change caused by the cytotoxic membrane perturbents suggests that TEER measurement would provide a simple and sensitive method for evaluating membrane-perturbing toxicants.

References

Oct 1, 1989·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·G Durand-CavagnaL R Gordon
Apr 1, 1995·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·J J van de SandtA A Rutten
Jan 31, 1997·Biochimica Et Biophysica Acta·K HashimotoM Shimizu
Feb 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·G DiogèneS Puiseux-Dao
Jun 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C GiovanniniM De Vincenzi
Feb 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A RossiM L Scarino
Apr 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J M GeeA H Penninks
Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F Zucco

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Citations

Jan 16, 2004·Archives of Pharmacal Research·So Yean ChoYeong Shik Kim
May 12, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T OkadaM Shimizu
Aug 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·G VelardeJ M Porcher
Aug 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·G VelardeJ M Porcher
Jun 11, 2011·Particle and Fibre Toxicology·Ece A MutluGökhan M Mutlu
Feb 9, 2000·Bioscience, Biotechnology, and Biochemistry·M KimM Shimuzu
Oct 1, 2005·Bioscience, Biotechnology, and Biochemistry·Atsushi Yokomizo, Masamitsu Moriwaki
Feb 9, 2010·Bioscience, Biotechnology, and Biochemistry·Makoto Shimizu
Feb 19, 2013·Advanced Drug Delivery Reviews·Jennifer M Gamboa, Kam W Leong
Feb 19, 2015·Journal of Pharmaceutical Sciences·Laís Cristina Da SilvaKênnia Rocha Rezende
Oct 31, 2012·Journal of Pharmaceutical Sciences·Driton VllasaliuSnow Stolnik
Dec 23, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Kesinee NetsomboonAndreas Bernkop-Schnürch
Apr 17, 2015·Advanced Healthcare Materials·Xin ZhaoAli Khademhosseini
Jan 22, 2009·Journal of Pharmaceutical Sciences·Siva Ram Kiran VakaS Narasimha Murthy
Feb 27, 2004·Biochemical and Biophysical Research Communications·Masashi TsukazakiMakoto Shimizu
Dec 12, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Carmen Frontela-SasetaGaspar Ros-Berruezo
Aug 3, 2000·Bioscience, Biotechnology, and Biochemistry·K IshizukaM Shimizu
Nov 27, 2004·Bioscience, Biotechnology, and Biochemistry·Asako NaraiMakoto Shimizu
Aug 22, 2016·Advanced Drug Delivery Reviews·Silvia Gabriella CiappellanoFederico Benetti
Nov 7, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Martina CirliniChiara Dall'Asta
Mar 2, 2018·Food & Function·Fabiola Moreno-OlivasGretchen J Mahler
Oct 27, 2017·Environmental Toxicology·Flávia Amadeu de OliveiraJ Michael Edwardson
Feb 14, 2012·Nature Nanotechnology·Gretchen J MahlerMichael L Shuler
Jul 25, 2013·Biofabrication·David Y S ChauAmir M Ghaemmaghami
May 12, 2020·Frontiers in Molecular Biosciences·Sugandha BhatiaErik W Thompson
Jul 3, 2019·Journal of Clinical Medicine·Sagar S Varankar, Sharmila A Bapat
Mar 25, 2011·The Journal of Nutrition·Dulantha UlluwishewaNicole C Roy
Dec 7, 2018·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Fabiola Moreno-OlivasGretchen J Mahler
Jul 20, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Andreia AlmeidaBruno Sarmento
Sep 18, 2003·Journal of Agricultural and Food Chemistry·Wichittra DaengprokYoshinori Mine

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