Rapid degradation of the tetrameric Mn cluster in illuminated, PsbO-depleted photosystem II preparations

Biochemistry. Biokhimii︠a︡
Boris K SeminA B Rubin

Abstract

A "decoupling effect" (light-induced electron transport without O2 evolution) was observed in Ca-depleted photosystem II (PSII(-Ca)) membranes, which lack PsbP and PsbQ (Semin et al. (2008) Photosynth. Res., 98, 235-249). Here PsbO-depleted PSII (PSII(-PsbO)) membranes (which also lack PsbP and PsbQ) were used to examine effects of PsbO on the decoupling. PSII(-PsbO) membranes do not reduce the acceptor 2,6-dichlorophenolindophenol (DCIP), in contrast to PSII(-Ca) membranes. To understand why DCIP reduction is lost, we studied light effects on the Mn content of PSII(-PsbO) samples and found that when they are first illuminated, Mn cations are rapidly released from the Mn cluster. Addition of an electron acceptor to PSII(-PsbO) samples accelerates the process. No effect of light was found on the Mn cluster in PSII(-Ca) membranes. Our results demonstrate that: (a) the oxidant, which directly oxidizes an as yet undefined substrate in PSII(-Ca) membranes, is the Mn cluster (not the Y(Z) radical or P680(+)); (b) light causes rapid release of Mn cations from the Mn cluster in PSII(-PsbO) membranes, and the mechanism is discussed; and (c) rapid degradation of the Mn cluster under illumination is significant for understanding the lack ...Continue Reading

Citations

Feb 6, 2016·Journal of Bioenergetics and Biomembranes·Boris K Semin, Michael Seibert
Oct 16, 2013·The Plant Journal : for Cell and Molecular Biology·Wenjuan WuJirong Huang
Dec 5, 2012·Chemical & Pharmaceutical Bulletin·Luiz Fabrício Gardini BrandãoNeli Kika Honda
Nov 5, 2020·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Elena R LovyaginaBoris K Semin
Nov 21, 2017·Journal of Photochemistry and Photobiology. B, Biology·B К SeminA B Rubin

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