Rapid degranulation of NK cells following activation by HIV-specific antibodies

The Journal of Immunology : Official Journal of the American Association of Immunologists
Amy W ChungIvan Stratov

Abstract

Ab-dependent cellular cytotoxicity (ADCC) Abs stimulate NK cell effector functions and play a role in protecting from and controlling viral infections. We characterized ADCC Abs in a cross-sectional cohort of 80 HIV-infected subjects not on antiretroviral therapy. We analyzed ADCC response by killing fluorescently labeled target cells, as well as expression of IFN-gamma and the degranulation marker CD107a from activated NK cells as measured by a novel intracellular cytokine assay. HIV-specific ADCC directed toward Envelope proteins were present in the majority of 80 untreated HIV-infected individuals measured by killing function. Similarly, most subjects had HIV-specific Abs that mediated degranulation or cytokine expression by NK cells. Interestingly, there was a poor correlation between ADCC-mediated killing of fluorescently labeled whole Envelope protein-pulsed cell lines and Ab-mediated expression of IFN-gamma by NK cells. However, in contrast to healthy donor NK cells, autologous patient NK cells more effectively degranulated granzyme B in response to ADCC activation. Activation of NK cells in response to stimulation by HIV-specific Abs occurs at least as rapidly as activation of Gag-specific CTLs. Our studies highlight th...Continue Reading

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Citations

Apr 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Amy W ChungIvan Stratov
Dec 10, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Matthew S ParsonsStephen J Kent
Jun 16, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Milla R McLeanStephen J Kent
Nov 16, 2017·Immunology·Kelly B Arnold, Amy W Chung
Aug 1, 2020·Frontiers in Immunology·Kimone Leigh FisherDerseree Archary
Nov 22, 2012·Expert Review of Clinical Immunology·Vijaya MadhaviIvan Stratov
Aug 30, 2014·The Journal of Infectious Diseases·Vijaya MadhaviStephen J Kent
Dec 27, 2016·Frontiers in Immunology·Austin W BoeschMargaret E Ackerman
Sep 28, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Diego A Vargas-InchausteguiMarjorie Robert-Guroff
Jan 10, 2018·Biotechnology and Bioengineering·Austin W BoeschMargaret E Ackerman
Mar 9, 2018·Journal of Virology·Anne B KristensenMatthew S Parsons
Jan 11, 2020·Frontiers in Immunology·Milla R McLeanAmy W Chung
Jul 28, 2011·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Amy W ChungIvan Stratov
Jun 3, 2009·The Journal of Experimental Medicine·Nilu GoonetillekeAndrew J McMichael
Aug 9, 2016·AIDS Research and Human Retroviruses·Gamze IsitmanNicole F Bernard
Mar 3, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Justin PollaraGuido Ferrari
May 28, 2019·Frontiers in Immunology·George K LewisBargavi Thyagarajan
Apr 1, 2011·Viral Immunology·Amy W ChungIvan Stratov
Jul 8, 2011·Clinical and Vaccine Immunology : CVI·Vladimir V MuruginMikhail V Pashenkov
Aug 24, 2012·Expert Review of Vaccines·Sorachai NitayaphanJean-Louis Excler
Nov 14, 2014·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Sanne S JensenIngrid Karlsson
Jul 8, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Justin PollaraGuido Ferrari

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