Rapid deployment of a mobile biosafety level-3 laboratory in Sierra Leone during the 2014 Ebola virus epidemic

PLoS Neglected Tropical Diseases
Yi ZhangZhendong Guo

Abstract

Ebola virus emerged in West Africa in December 2013. The high population mobility and poor public health infrastructure in this region led to the development of the largest Ebola virus disease (EVD) outbreak to date. On September 26, 2014, China dispatched a Mobile Biosafety Level-3 Laboratory (MBSL-3 Lab) and a well-trained diagnostic team to Sierra Leone to assist in EVD diagnosis using quantitative real-time PCR, which allowed the diagnosis of suspected EVD cases in less than 4 hours from the time of sample receiving. This laboratory was composed of three container vehicles equipped with advanced ventilation system, communication system, electricity and gas supply system. We strictly applied multiple safety precautions to reduce exposure risks. Personnel, materials, water and air flow management were the key elements of the biosafety measures in the MBSL-3 Lab. Air samples were regularly collected from the MBSL-3 Lab, but no evidence of Ebola virus infectious aerosols was detected. Potentially contaminated objects were also tested by collecting swabs. On one occasion, a pipette tested positive for EVD. A total of 1,635 suspected EVD cases (824 positive [50.4%]) were tested from September 28 to November 11, 2014, and no membe...Continue Reading

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Mar 21, 2019·Human Vaccines & Immunotherapeutics·Trina Racine, Gary P Kobinger
May 8, 2020·The American Journal of Forensic Medicine and Pathology·J Matthew LacySuzanne Utley
Oct 22, 2020·Biochemical Society Transactions·Zhongyi WangQiang Sun
Jan 13, 2021·Trends in Biotechnology·Richard I KitneyJim Philp
May 14, 2021·Clinical Microbiology Reviews·Carmen L CharltonEric P Bind
Jun 25, 2021·Frontiers in Cellular and Infection Microbiology·Cheng ZhangShishan Dong
Aug 1, 2021·The Lancet Infectious Diseases·Carrie M Long, Andrea Marzi

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