Rapid depot-specific activation of adipocyte precursor cells at the onset of obesity

Nature Cell Biology
Elise JefferyMatthew S Rodeheffer

Abstract

Excessive accumulation of white adipose tissue (WAT) is the defining characteristic of obesity. WAT mass is composed primarily of mature adipocytes, which are generated through the proliferation and differentiation of adipocyte precursors (APs). Although the production of new adipocytes contributes to WAT growth in obesity, little is known about the cellular and molecular mechanisms underlying adipogenesis in vivo. Here, we show that high-fat diet feeding in mice rapidly and transiently induces proliferation of APs within WAT to produce new adipocytes. Importantly, the activation of adipogenesis is specific to the perigonadal visceral depot in male mice, consistent with the patterns of obesogenic WAT growth observed in humans. Furthermore, we find that in multiple models of obesity, the activation of APs is dependent on the phosphoinositide 3-kinase (PI3K)-AKT2 pathway; however, the development of WAT does not require AKT2. These data indicate that developmental and obesogenic adipogenesis are regulated through distinct molecular mechanisms.

References

Jul 1, 1976·Clinics in Endocrinology and Metabolism·J Hirsch, B Batchelor
Jul 5, 2003·The Journal of Clinical Investigation·Robert S GarofaloKevin G Coleman
Aug 1, 1962·The Journal of Pediatrics·M D STEINBERGA ANGEL
Jun 1, 1962·The Journal of Nutrition·S C PECKHAMH W CARROLL
Mar 10, 2005·The American Journal of Clinical Nutrition·Youfa WangFrank B Hu
Sep 18, 2007·Genesis : the Journal of Genetics and Development·Mandar Deepak MuzumdarLiqun Luo
Dec 14, 2007·Annual Review of Genetics·Jason Pellettieri, Alejandro Sánchez Alvarado
Jan 29, 2008·Physiology & Behavior·N A van Herpen, V B Schrauwen-Hinderling
May 6, 2008·Nature·Kirsty L SpaldingPeter Arner
May 8, 2008·Cell Metabolism·Thien T TranC Ronald Kahn
May 14, 2008·Current Hypertension Reports·Liza K Phillips, Johannes B Prins
Jun 4, 2008·Annual Review of Biochemistry·Peter Tontonoz, Bruce M Spiegelman
Jul 9, 2008·International Journal of Obesity : Journal of the International Association for the Study of Obesity·T KellyJ He
Oct 7, 2008·Cell·Matthew S RodehefferJeffrey M Friedman
Jul 15, 2009·Cell Cycle·Eva Gonzalez, Timothy E McGraw
Aug 22, 2009·Annual Review of Genetics·Ville Hietakangas, Stephen M Cohen
Nov 4, 2009·Cell Metabolism·Karla F LeavensMorris J Birnbaum
Nov 26, 2009·Plastic and Reconstructive Surgery·Hitomi EtoKotaro Yoshimura
Dec 23, 2009·Nature Reviews. Molecular Cell Biology·Laura R PearceDario R Alessi
Jan 30, 2010·Obesity·Yuji YamamotoC Ronald Kahn
Feb 17, 2010·International Journal of Obesity : Journal of the International Association for the Study of Obesity·K L GroveD J Clegg
Jun 3, 2011·The Journal of Clinical Investigation·Laurent Gautron, Joel K Elmquist
Sep 29, 2011·Nature Reviews. Molecular Cell Biology·Ana G Cristancho, Mitchell A Lazar
Oct 8, 2011·Science·K HussainR K Semple
Oct 27, 2011·Development·Jinah HanGou Young Koh
Feb 26, 2013·Nature Cell Biology·Ryan Berry, Matthew S Rodeheffer
Feb 28, 2013·Diabetologia·M P CzechM Aouadi
Apr 2, 2013·The Journal of Clinical Endocrinology and Metabolism·Peter ArnerMikael Rydén
Nov 19, 2013·Cell Metabolism·Ryan BerryMatthew S Rodeheffer
Jan 21, 2014·Cell·Evan D Rosen, Bruce M Spiegelman
Feb 1, 2014·Methods in Enzymology·Christopher D ChurchMatthew S Rodeheffer
Feb 1, 2014·Methods in Enzymology·Ryan BerryMatthew S Rodeheffer
Jul 30, 2014·Adipocyte·Elise JefferyMatthew S Rodeheffer
Dec 3, 2014·Stem Cell Reports·Katherine C KruegerBrian J Feldman

❮ Previous
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Citations

Jan 21, 2016·International Journal of Endocrinology·Myrte Merkestein, Dyan Sellayah
Nov 26, 2015·Seminars in Immunology·Felix M WensveenBojan Polić
Feb 2, 2016·American Journal of Obstetrics and Gynecology·Amanda S Janesick, Bruce Blumberg
Jul 30, 2015·European Journal of Immunology·Felix M WensveenBojan Polić
Apr 5, 2016·Diabetologia·Vanessa PellegrinelliAntonio Vidal-Puig
Jul 21, 2015·Cell Metabolism·Mikael RydénPeter Arner
Aug 19, 2015·Nature Cell Biology·Qiong A WangPhilipp E Scherer
Feb 26, 2016·Reviews in Endocrine & Metabolic Disorders·Vasileia Ismini Alexaki, Triantafyllos Chavakis
Jun 21, 2016·Cell Metabolism·Elise JefferyMatthew S Rodeheffer
Jul 8, 2016·Thrombosis and Haemostasis·Dries BautersH Roger Lijnen
May 6, 2016·Annual Review of Cell and Developmental Biology·Brett ShookValerie Horsley
Oct 18, 2016·Cell Stem Cell·Guillermo C Rivera-GonzalezValerie Horsley
Oct 21, 2016·Reproductive Toxicology·Jerrold J HeindelFrederick Vom Saal
Oct 26, 2016·Scientific Reports·Wensi TaoXiang-Xi Xu
Oct 30, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yoon Hee LeeHocheol Kim
Jan 5, 2017·Cell Reports·Blandine SeccoMathieu Laplante
Feb 17, 2017·Genes & Development·Chelsea HeplerRana K Gupta
Apr 25, 2017·Nature Cell Biology·Yanhui ZhangRajan Sah
Jun 4, 2016·Cell Stem Cell·Matthew S Rodeheffer, Mark C Horowitz
Jun 15, 2017·International Journal of Obesity : Journal of the International Association for the Study of Obesity·A CarrièreL Casteilla
Mar 9, 2018·The Journal of Experimental Biology·Theresa SchoettlSiegfried Ussar
Nov 16, 2017·Scientific Reports·Giorgia MaroniDmitry Penkov
Nov 24, 2016·American Journal of Physiology. Endocrinology and Metabolism·Yuanyuan WuSusan K Fried
Mar 10, 2018·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·F LouwenJ Yuan

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Methods Mentioned

BETA
confocal microscopy
flow cytometry
Protein Assay
NMR
pull down
PCR

Software Mentioned

Image J
GraphPad
BD FACS Diva
GraphPad Prism
ImageJ

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