Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I

The Journal of Veterinary Medical Science
Cintia Hiromi OkinoH J Montassier

Abstract

A method based on Melting Temperature analysis of Hypervariable regions (HVR) of S1 gene within a RT-qPCR was developed to detect different genotypes of avian infectious bronchitis virus (IBV) and identify the Mass genotype. The method was able to rapidly identify the Mass genotype among IBV field isolates, vaccine attenuated strains and reference M41 strain in allantoic liquid and also directly in tissues. The RT-qPCR developed detected the virus in both tracheal and pulmonary samples from M41-infected or H120-infected birds, in a larger post-infection period compared to detection by standard method of virus isolation. RT-qPCR method tested provided a sensitivity and rapid approach for screening on IBV detection and Mass genotyping from IBV isolates.

References

Jun 9, 2005·Journal of Virological Methods·Salin ChutinimitkulYong Poovorawan
Jun 19, 2012·Avian Pathology : Journal of the W.V.P.A·Jane K A CookR C Jones
Feb 13, 2013·Avian Diseases·Mark W Jackwood
Sep 17, 2014·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Shr-Wei HuangChi-Young Wang

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Citations

Mar 7, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Salman L ButtJames B Stanton
Mar 27, 2021·Journal of Virological Methods·M S Abd El HafezS M Ibrahim

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Datasets Mentioned

BETA
AY851295

Methods Mentioned

BETA
genotyping
electrophoresis
PCR

Software Mentioned

EVAGREEN

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