Rapid detection and immune characterization of Mycobacterium abscessus infection in cystic fibrosis patients

PloS One
Mathis SteindorMarc Jacobsen

Abstract

Cystic fibrosis patients are highly susceptible to infections with non-tuberculous mycobacteria. Especially Mycobacterium abscessus infections are common but reliable diagnosis is hampered by non-specific clinical symptoms and insensitive mycobacterial culture. In the present study we established novel methods for rapid detection and immune characterization of Mycobacterium abscessus infection in cystic fibrosis patients. We performed Mycobacterium abscessus specific DNA-strip- and quantitative PCR-based analyses of non-cultured sputum samples to detect and characterize Mycobacterium abscessus infections. Concomitantly in vitro T-cell reactivation with purified protein derivatives (PPDs) from different mycobacterial species was used to determine Mycobacterium abscessus specific T-cell cytokine expression of infected cystic fibrosis patients. Four of 35 cystic fibrosis patients (11.4%) were Mycobacterium abscessus culture positive and showed concordant DNA-strip-test results. Quantitative PCR revealed marked differences of mycobacterial burden between cystic fibrosis patients and during disease course. Tandem-repeat analysis classified distinct Mycobacterium abscessus strains of infected cystic fibrosis patients and excluded pat...Continue Reading

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Citations

Oct 13, 2019·Applied and Environmental Microbiology·Maura J DonohueJoyce M Donohue
Mar 7, 2019·Frontiers in Immunology·Getahun AbateDaniel F Hoft

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Methods Mentioned

BETA
PCR
electrophoresis
FCS
DNA-strip
flow cytometry

Software Mentioned

SigmaStat Systat
FCS Express

Related Concepts

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