Rapid detection and sequence-specific differentiation of extended-spectrum beta-lactamase GES-2 from Pseudomonas aeruginosa by use of a real-time PCR assay

Antimicrobial Agents and Chemotherapy
Gerhard F Weldhagen

Abstract

The LightCycler was compared to nested PCR for the detection of bla(GES/IBC) genes from 100 Pseudomonas aeruginosa clinical isolates. The real-time PCR assay detected a bla(GES/IBC) gene product from 83 isolates, exhibiting a sensitivity and specificity of 94.3 and 100% respectively, compared to nested PCR and DNA sequencing.

References

Oct 23, 1997·Antimicrobial Agents and Chemotherapy·L N PhilipponP Nordmann
Nov 11, 1999·International Journal of Systematic Bacteriology·L HaubenJ Swings
Jun 20, 2000·Antimicrobial Agents and Chemotherapy·J Kim, H J Lee
Aug 18, 2000·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C NiederhauserI Heinzer
May 17, 2001·Antimicrobial Agents and Chemotherapy·C C Randegger, H Hächler
Aug 15, 2001·Antimicrobial Agents and Chemotherapy·L PoirelP Nordmann
Jul 25, 2003·Antimicrobial Agents and Chemotherapy·Gerhard F WeldhagenPatrice Nordmann
Dec 23, 2003·The Journal of Antimicrobial Chemotherapy·Mi-Kyung LeeBeth A Arthington-Skaggs
Jan 13, 2004·Journal of Clinical Microbiology·Petra WolffsPeter Rådström
Jun 15, 2004·International Journal of Antimicrobial Agents·Gerhard F Weldhagen

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Citations

Jul 12, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·F DonnarummaE Casalone
Nov 8, 2006·Antimicrobial Agents and Chemotherapy·Thierry NaasPatrice Nordmann
Aug 31, 2006·Antimicrobial Agents and Chemotherapy·Xiaofei JiangYuan Lu
Aug 8, 2006·Journal of Clinical Microbiology·Laurent PoirelPatrice Nordmann
Jun 1, 2006·The Annals of Pharmacotherapy·Kathryn E SabolJames S Lewis
Jan 15, 2010·The Annals of Pharmacotherapy·Karyn M Sullivan, Linda M Spooner
Dec 2, 2005·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Natali AzizWilliam E Benitz

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