PMID: 9646003Jul 1, 1998Paper

Rapid detection, culture-amplification and typing of herpes simplex viruses by enzyme immunoassay in clinical samples

Clinical and Diagnostic Virology
T KokS K Schepetiuk

Abstract

The laboratory diagnosis of herpes simplex infection may require rapid (direct) tests, as well as cell cultures, for detection of the virus in clinical samples. The quantity of virus present in clinical samples is variable and this may depend on the period from onset of rash. In addition, not all patients may show obvious symptoms with this infection. The successful culture of herpes simplex virus requires prompt transportation after collection of the specimen as the virus is easily inactivated. Hence, rapid and culture tests would enable detection of non-viable and viable viruses. We describe the rapid detection of HSV by EIA directly in various clinical samples using commercially available polyclonal sera. In addition specimens were inoculated in microwell cell cultures and 4 days post inoculation the culture fluids were tested for HSV and subtyped by a similar EIA (culture amplified EIA). The direct EIA showed an endpoint detection of 100 TCID50/ml, sensitivity of 92% (all specimen types) and specificity of 100%. The direct EIA sensitivity was 97% in non-genital specimens and 88% in genital specimens. The culture amplified EIA showed a sensitivity of 95% compared to all confirmed HSV positive samples. The results of the HSV ...Continue Reading

References

Nov 1, 1977·The Journal of General Virology·R A KillingtonJ Mumford
Jun 4, 1992·The New England Journal of Medicine·L A KoutskyL Corey
May 1, 1985·Journal of Clinical Pathology·I AlexanderJ M Darville
Jul 1, 1986·Journal of Medical Virology·V SchusterD Neumann-Haefelin
Feb 1, 1997·Current Opinion in Pediatrics·D E Dwyer, A M Kesson

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Citations

Feb 19, 2000·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·T KokG Higgins
Aug 2, 2006·Journal of Virological Methods·Hikmet Murat SipahiogluMustafa Ocak
Aug 2, 2021·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Tekumalla SindhujaSomesh Gupta
Feb 3, 2000·Journal of Clinical Microbiology·M J EspyT F Smith

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