PMID: 19923484DOI: 10.1128/jcm.01498-09Nov 20, 2009Paper
Rapid detection of aac(6')-Ib-cr quinolone resistance gene by pyrosequencing.
Journal of Clinical Microbiology
Thomas GuillardC De Champs
Abstract
Pyrosequencing was used to rapidly detect aac(6')-Ib and aac(6')-Ib-cr genes. This plasmid-mediated quinolone resistance determinant is increasing in extended-spectrum beta-lactamase-producing Enterobacteriaceae. This method is faster and more cost-effective than the methods previously described. Sequences obtained with this pyrosequencing method showed 100% concordance with conventional sequencing.
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