PMID: 9524322Apr 3, 1998Paper

Rapid detection of Actinobacillus actinomycetemcomitans, Prevotella intermedia and Porphyromona gingivalis by multiplex PCR

Journal of Periodontal Research
L GarcíaMariano Sanz

Abstract

The identification of specific periodontal pathogens by conventional methods, mainly anaerobic cultivation, is difficult, time consuming and even sometimes unreliable. Therefore, a multiplex PCR method for simultaneous detection of Actinobacillus actinomycetemcomitans (A.a.), Porphyromona gingivalis (P.g.) and Prevotella intermedia (P.i.) was developed for rapid and easy identification of these specific bacterial pathogens in subgingival plaque samples. In this paper, there is a detailed description of the oligonucleotide primer selection, DNA extraction and PCR conditions and the sequencing of the amplified products. The locus chosen to be amplified is a highly variable region in the 16S ribosomal DNA. For the development of this technique ATCC cultures and pure cultures from subgingival plaque samples taken from periodontitis patients were used. As an internal positive control a recombinant plasmid was developed. This simple DNA extraction procedure and the DNA amplification and visualization of the amplified product permits the detection of the bacteria in a working day. Thus, this multiplex PCR method is a rapid and effective detection method for specific periodontal pathogens.

References

Dec 1, 1992·Journal of Periodontology·W J Loesche
Apr 1, 1992·Journal of Periodontology·S S Socransky, A D Haffajee
Jan 1, 1991·Proteins·G D SchulerD J Lipman
Jan 1, 1990·Archives of Oral Biology·R Gmür, B Guggenheim
Jan 1, 1990·Archives of Oral Biology·E D SavittW J Peros
Nov 1, 1986·Oral Microbiology and Immunology·J J ZambonR J Genco
Mar 1, 1987·Molecular and Cellular Probes·R P Viscidi, R G Yolken
Dec 1, 1992·Journal of Periodontology·Walter J Loesche

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Citations

Mar 28, 2009·Journal of Oral Science·Shivaprasad BilichodmathKishore Bhat
Nov 3, 2009·Periodontology 2000·Bruce J Paster, Floyd E Dewhirst
Nov 24, 2016·Clinical and Experimental Dental Research·Masaaki TakeshitaFusanori Nishimura
Aug 11, 2016·Clinical and Experimental Dental Research·Joshua CoffeyVineet K Singh
Jan 30, 2019·Clinical Oral Investigations·Robert DurandElham Emami
Dec 10, 2020·Journal of Medical Microbiology·Ioannis FragkioudakisDanae Anastasia Apatzidou
Oct 12, 2000·Clinical Microbiology Reviews·E M ElnifroP E Klapper

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