PMID: 9544976Apr 17, 1998Paper

Rapid detection of anticardiolipin antibodies

American Journal of Hematology
M W StewartP A Gordon

Abstract

A rapid screening method for the detection of antiphospholipid antibodies is described. Dense, red dyed polystyrene beads coated with cardiolipin were incubated with test sera for a short period of time, then added to a microtube containing anti-human IgG in a gel provided within a pre-cast card (DiaMed ID Microtyping System). The card was centrifuged at 150g for 5 min and then examined for movement of the beads through the gel. Beads without bound antibody travelled through the gel and formed a pellet on the bottom of the tube. Anti-human IgG within the gel matrix impeded cardiolipin-coated beads when antiphospholipid antibodies bound to the beads. Positivity was indicated by the formation of a layer of beads on the top of the gel matrix. Prospective analysis of 103 samples for the presence of antiphospholipid antibodies by flow cytometry and the gel-card technique showed good correlation between the two methods. All samples found to be positive by flow cytometry (23 of 103) were identified as positive by the gel-card technique. Two samples were identified as positive by the gel-card method but negative by flow cytometry. The technique is simple to perform and should prove useful as a rapid screening method for the detection o...Continue Reading

References

Oct 1, 1992·American Journal of Reproductive Immunology : AJRI·D A Triplett
Sep 1, 1993·British Journal of Haematology·S S PierangeliW L Dean

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Citations

Sep 11, 2004·International Journal of Dermatology·Maria José Nogueira DiógenesRosangela R A Holanda
Apr 25, 2007·Critical Reviews in Clinical Laboratory Sciences·P G VlachoyiannopoulosP Tsiligros

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Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.