Rapid detection of Aspergillus flavus in rice using biofunctionalized carbon nanotube field effect transistors

Analytical and Bioanalytical Chemistry
Raquel A VillamizarF Xavier Rius

Abstract

In the present study, we have used carbon nanotube field effect transistors (FET) that have been functionalized with protein G and IgG to detect Aspergillus flavus in contaminated milled rice. The adsorbed protein G on the carbon nanotubes walls enables the IgG anti-Aspergillus antibodies to be well oriented and therefore to display full antigen binding capacity for fungal antigens. A solution of Tween 20 and gelatine was used as an effective blocking agent to prevent the non-specific binding of the antibodies and other moulds and also to protect the transducer against the interferences present in the rice samples. Our FET devices were able to detect at least 10 μg/g of A. flavus in only 30 min. To evaluate the selectivity of our biosensors, Fusarium oxysporum and Penicillium chrysogenum were tested as potential competing moulds for A. flavus. We have proved that our devices are highly selective tools for detecting mycotoxigenic moulds at low concentrations in real samples.

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Jul 28, 2009·Biosensors & Bioelectronics·Raquel A VillamizarF Xavier Rius

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Citations

Sep 8, 2011·Analytical and Bioanalytical Chemistry·Leslie R Farris, Melisenda J McDonald
May 8, 2016·Talanta·Fernando Cámara-MartosTeresa Rocha-Santos
Jan 12, 2021·Journal of the Science of Food and Agriculture·Shuang GuJun Wang
Nov 6, 2020·Analytical Chemistry·Rebeca S RodriguezChristy L Haynes

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Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

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