Abstract
Candida species, especially C. albicans, are commensals on human mucosal surfaces, but are increasingly becoming one of the important invasive pathogens as seen by a rise in its prevalence in immunocompromised patients and in antibiotic consumption. Thus, an accurate identification of Candida species in patients with pulmonary symptoms can provide important information for effective treatment. A total of 75 clinical isolates of Candida species were obtained from the bronchoalveolar lavage fluid of both immunocompromised and immunocompetent patients with pulmonary symptoms. Candida cultures were identified based on nuclear ribosomal Internal Transcribed Spacer (ITS1-ITS2 rDNA) sequence analysis by polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP). Molecular identification indicated that the isolates belonged predominantly to C. albicans (52%), followed by C. tropicalis (24%), C. glabrata (14.7%), C. krusei (5.3%), C. parapsilosis (1.3%), C. kefyr (1.3%) and C. guilliermondii (1.3%). Given the increasing complexity of disease profiles and their management regimens in diverse patients, rapid and accurate identification of Candida species can lead to timely and appropriate antifungal therapy.
References
Dec 1, 1977·The American Journal of Medicine·H MasurD Armstrong
Apr 1, 1980·American Journal of Clinical Pathology·T E KiehnD Armstrong
May 1, 1993·The Journal of Infectious Diseases·C Beck-Sagué, W R Jarvis
Nov 1, 1995·Comprehensive Therapy·M G Cormican, M A Pfaller
Nov 1, 1996·Journal of Clinical Microbiology·J S HeelanK Coon
Jan 1, 1997·Antimicrobial Agents and Chemotherapy·F S NolteJ R Wingard
Aug 1, 1997·American Journal of Respiratory and Critical Care Medicine·M el-EbiaryM T Jiménez de Anta
Apr 23, 1999·Diagnostic Microbiology and Infectious Disease·M A PfallerR A Hajjeh
Feb 19, 2000·Antimicrobial Agents and Chemotherapy·M A PfallerR J Hollis
Jan 5, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D P KontoyiannisI I Raad
Apr 6, 2002·American Journal of Respiratory and Critical Care Medicine·Jean Chastre, Jean-Yves Fagon
Aug 8, 2002·Oral Diseases·L P SamaranayakeP Wanzala
Dec 12, 2002·Bone Marrow Transplantation·A Safdar, D Armstrong
Jan 7, 2003·Infectious Disease Clinics of North America·Jose A Vazquez, Jack D Sobel
May 19, 2004·Intensive Care Medicine·Elie AzoulayJean-François Timsit
Aug 3, 2006·Medical Mycology·Andrew M BormanElizabeth M Johnson
Aug 31, 2006·Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology·Hossein MirhendiHideyo Yamaguchi
Apr 10, 2009·Intensive Care Medicine·W MeerssemanE Van Wijngaerden
Jan 9, 2010·Journal of Medical Microbiology·Hossein MirhendiMaiken Cavling Arendrup
Aug 20, 2010·Medical Mycology·Nuri Kiraz, Yasemin Oz
May 17, 2011·FEMS Microbiology Reviews·Sónia SilvaJoana Azeredo
Jun 10, 2011·Indian Journal of Medical Microbiology·S AgarwalP Bhalla
Mar 9, 2013·Medical Mycology·Rasoul MohammadiKoichi Makimura
Citations
Oct 28, 2017·Journal of Clinical Laboratory Analysis·Alireza EsmailzadehTanuka Sen
Sep 26, 2019·Journal of Medical Microbiology·Lingyue SunJin Yu
Apr 20, 2017·Pathogens and Disease·Kathryn M PendletonRobert P Dickson
Jun 12, 2018·BioMed Research International·Saeid Mahdavi OmranMojtaba Taghizadeh-Armaki
Dec 5, 2019·Antibiotics·Antonia Thassya Lucas Dos SantosHenrique Douglas Melo Coutinho
Jan 23, 2018·Current Medical Mycology·A KamariR Mohammadi
Nov 17, 2019·Pathogens·Kathy MontesGustavo Fontecha
Apr 16, 2021·Frontiers in Medicine·Yirui XieBiao Zhu
Mar 28, 2020·Heliyon·Shirin FarahyarMaryam Rahimi