Rapid detection of carbapenemase activity of Enterobacteriaceae isolated from positive blood cultures by MALDI-TOF MS

Annals of Clinical Microbiology and Antimicrobials
Jiajia YuLisong Shen

Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been proved to be a useful tool for identification of pathogens directly isolated from blood cultures in clinical microbiology laboratories. β-Lactam antibiotics are commonly used for treatment of bloodstream infections caused by Enterobacteriaceae strains, and carbapenem is the superlative class of β-lactam antibiotics. Since the carbapenem resistance rate of Enterobacteriaceae strains raised year by year, efficient detection of carbapenemase activity and timely delivery of carbapenem susceptibility reports of Enterobacteriaceae strains isolated from blood cultures is important for clinicians. We used 64 simulated blood cultures to establish the method of MALDI-TOF MS based ertapenem hydrolysis assay. The cutoff value of logRQ calculated from the peaks intensity of ertapenem and its hydrolysate was first set to identify the strains with carbapenemase activity. Then, we detected and calculated the logRQ values of 385 Enterobacteriaceae strains from positive clinical blood cultures to distinguish the carbapenemase producers and noncarbapenemase producers. The mean logRQ value of 32 noncarbapenemase producers was - 0.85 ± 0.14 in simul...Continue Reading

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Citations

Jun 30, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Ioannis K Neonakis, Demetrios A Spandidos
Jun 27, 2019·Expert Review of Anti-infective Therapy·Adamantia LiapikouAntoni Torres
Sep 5, 2019·Frontiers in Microbiology·Xiaoyan CuiHong Du
Jan 7, 2021·Future Microbiology·Muhammad Usman QamarMark A Toleman
Oct 4, 2020·Current Opinion in Infectious Diseases·Abigail K KoisJoseph L Kuti
Jun 13, 2021·Journal of Microbiological Methods·Camila Mörschbächer WilhelmAfonso Luís Barth

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Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.