Rapid detection of cows' milk in sheeps' and goats' milk by a species-specific polymerase chain reaction technique

Journal of Dairy Science
Inés López-CallejaR Martín

Abstract

A polymerase chain reaction (PCR) assay was developed for the specific identification of cows' milk in sheep's and goats' milk by using primers targeting the mitochondrial 12S rRNA gene. The use of a forward primer complementary to a conserved DNA sequence, along with a reverse primer specific for cow, yielded a 223-bp fragment from cows' milk DNA, whereas no amplification signal was obtained in sheep's and goats' milk DNA. The technique was applied to raw, pasteurized, and sterilized milk binary mixtures of cow-sheep and cow-goat, enabling the specific detection of cows' milk with a good sensitivity threshold (0.1%). The proposed PCR assay represents a rapid and straightforward method applicable to the authentication of milk and other dairy products in routine analysis.

References

Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T D KocherA C Wilson
May 1, 1997·The Journal of Dairy Research·M AmillsA Sanchez
Nov 14, 1997·Journal of Applied Microbiology·R GutiérrezR Martín
Mar 15, 2000·Journal of Food Protection·R BranciariJ A Lenstra
Aug 16, 2001·The Journal of Dairy Research·C Maudet, P Taberlet
Feb 19, 2002·Journal of Food Protection·M T BotteroS Rosati
Dec 5, 2002·The Journal of Dairy Research·Michael A MurphyChris Moran
Mar 6, 2003·Journal of Agricultural and Food Chemistry·Miguel A RodríguezRosario Martín

❮ Previous
Next ❯

Citations

Feb 1, 2009·Nutrients·Elena PonzoniDiego Breviario
May 27, 2014·Journal of Environmental Health Science & Engineering·Mohammad RafieeMohammad Hajaghazadeh
Feb 14, 2014·Scientific Reports·Li YangRonghua Li
Nov 21, 2017·Journal of Dairy Science·Stephanie Clark, María Bárbara Mora García
Nov 7, 2017·Journal of Dairy Science·J LiaoY F Liu
Sep 9, 2017·Critical Reviews in Food Science and Nutrition·Caterina AgrimontiNelson Marmiroli
Apr 9, 2008·Journal of Food Protection·Irene MartínRosario Martín
Dec 19, 2012·Journal of Dairy Science·O GuerreiroM Duarte
Aug 19, 2005·Journal of Dairy Science·I López-CallejaR Martín
Sep 1, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Thomas HolzhauserSimon D Flanagan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.