PMID: 16613660Apr 15, 2006Paper

Rapid detection of epidermal growth factor receptor mutations in non-small cell lung cancer using real-time polymerase chain reaction with TaqMan-MGB probes

The Cancer Journal
Caicun ZhouBo Su

Abstract

We investigated somatic mutations of the epidermal growth factor receptor gene in non-small cell lung cancer tumor tissue and their detection using real-time polymerase chain reaction with TaqMan-MGB probes. The DNA was extracted from surgically resected non-small cell lung cancer tumor specimens. Genes encoding for epidermal growth factor receptor tyrosine (exons 18, 19, and 21) were amplified by nested polymerase chain reaction, sequenced, and analyzed by chromatograms with manual review. TaqMan-MGB probes were designed to detect the epidermal growth factor receptor gene mutations in the tumor tissues using real-time polymerase chain reaction. Somatic point mutations and deletions were identified in the tyrosine kinase domain of the epidermal growth factor receptor gene in 21 of 80 non-small cell lung cancer patients, including 13 patients with deletion mutations occurring in exon 19 and 8 patients with point mutations occurring in codon 858 (exon 21). The results from real-time polymerase chain reaction with TaqMan-MGB probes were completely consistent with sequencing outcomes. Both the sensitivity and specificity for detecting the epidermal growth factor receptor gene mutations using real-time polymerase chain reaction with...Continue Reading

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Citations

Aug 10, 2013·Journal of Experimental & Clinical Cancer Research : CR·Hye-Ryoun KimKye Young Lee
Jun 24, 2015·Journal of Assisted Reproduction and Genetics·Neng-neng ZhengRuo-heng Zheng
Oct 26, 2016·International Journal of Cardiology·Asma Amrani-MidounNabila Bouatia-Naji
Jun 24, 2008·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Marc Ladanyi, William Pao
Apr 15, 2006·The Cancer Journal·Adi F Gazdar
Jun 27, 2008·Advances in Anatomic Pathology·Sanja Dacic
Sep 5, 2014·The Cochrane Database of Systematic Reviews·Elvira C van DalenLeontien C M Kremer
Nov 9, 2019·International Journal of Clinical Oncology·Fangfang NieWanyang Liu
Sep 6, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kanako HoshiHiroshi Shimada
Sep 6, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·William Pao, Marc Ladanyi
Apr 30, 2011·Archives of Pathology & Laboratory Medicine·Sanja Dacic

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