Rapid Detection of Escherichia coli in Water Using Sample Concentration and Optimized Enzymatic Hydrolysis of Chromogenic Substrates

Current Microbiology
Jianyong WuJamie K Bartram

Abstract

Methods for rapid detection of fecal indicator bacteria in water are important to ensure that water is safe for drinking, bathing, recreation, fishing and shellfish harvesting. In this study, we tested experimental conditions for bacterial hydrolysis of two promising enzymatic substrates, 5-Bromo-4-chloro-3-indolyl β-D-glucuronide (X-Gluc) and Resorufin β-D-glucuronide (REG), and optimized parameters such as temperature and pH to determine conditions for rapid reactions. We then innovated a membrane filter-based approach to facilitate more rapid enzyme-based detection of Escherichia coli in water based on the combination of an initial concentration step and optimized test conditions. For this approach, a water sample (10‒100 mL) is filtered through a 0.45-µm pore size filter with a diameter of 4 or 13 mm. After filtration, a newly designed rapid detection broth is added containing the enzymatic inducer Methyl-beta-D-Glucuronide sodium (MetGlu) and the substrate REG or X-Gluc. After a few (1‒7) hours of incubation at 35 °C, the filter shows pink color (for REG-containing broth) or green color (for X-Gluc containing broth) if E. coli is present. The study provides insights and approaches towards developing a simple, fast, and low...Continue Reading

References

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Jan 19, 2016·Enzyme and Microbial Technology·Nikou HesariMorteza Abbaszadegan

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Citations

Apr 6, 2019·Journal of Environmental Quality·J Randall EtheridgeCharlie Humphrey
Nov 9, 2019·Analytical and Bioanalytical Chemistry·Ciprian Briciu-BurghinaFiona Regan
Jul 24, 2021·Biotechnology and Applied Biochemistry·Sebnem SeherlerIlke Anac Sakir

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Methods Mentioned

BETA
membrane filtration

Software Mentioned

SAS
MUG

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