Rapid Detection of Escherichia coli via Enzymatically Triggered Reactions in Self-Reporting Chitosan Hydrogels.

ACS Applied Materials & Interfaces
Mir-Morteza Sadat EbrahimiHolger Schönherr

Abstract

In this work, a self-reporting hydrogel for the rapid in situ detection of bacterial enzymes is reported. To implement the reporting function for the bacterium Escherichia coli into a film-based sensing format, chitosan hydrogel films on solid backing supports were equipped with a reporting function for the enzyme β-glucuronidase (β-GUS), which is secreted by >98% of all known E. coli strains. Covalent coupling of the fluorogenic substrate 4-methylumbelliferyl-β-D-glucuronide or the complementary chromogenic substrate 4-nitrophenyl-β-D-glucuronide via amide bond formation afforded an attachment that is stable for >24 h under physiological conditions. By contrast, in the presence of β-GUS, the reporter dyes were very rapidly cleaved and produced a signal for the presence of the enzyme, which was detectable by bare eye under appropriate illumination. Detailed investigations of the enzymatic reaction for both types of substrates in neat enzyme solution as well as in bacterial supernatant revealed the apparent reaction kinetics and allowed us to determine the concentration of β-GUS in the supernatant. Under optimized conditions, the 4-methylumbelliferyl-β-D-glucuronide-functionalized hydrogel reported the presence of β-GUS within 1...Continue Reading

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Citations

Sep 22, 2017·Current Medicinal Chemistry·Antonio FranceskoTzanko Tzanov
Nov 16, 2019·Beilstein Journal of Organic Chemistry·Aydan YadigarliHolger Schönherr
Aug 26, 2020·Macromolecular Rapid Communications·Hang ZhangMingfeng Wang
Jan 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yangcui QuHong Chen
Apr 4, 2017·Advanced Healthcare Materials·Il Young JungHyukjin Lee
Nov 24, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Pengfei LiShiyong Zhang
Nov 21, 2020·Angewandte Chemie·Christina M GeiselhartChristopher Barner-Kowollik
Jul 31, 2020·Biosensors & Bioelectronics·Maha AlafeefDipanjan Pan
Dec 20, 2017·Journal of Agricultural and Food Chemistry·Hongxia WangFuyuan Ding

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