PMID: 7462410Jan 1, 1981

Rapid detection of Gram-negative bacteriuria by Limulus amoebocyte lysate assay

Journal of Clinical Microbiology
R Nachum, E Shanbrom


The Limulus amoebocyte lysate (LAL) test was evaluated for rapid detection of gram-negative bacteriuria in an adult patient population. Time to gelation of a standard LAL preparation was used as a measure of significant (greater than 10(5) bacteria per ml) gram-negative bacteriuria, and the results of 190 LAL assays were compared with quantitative urine cultures. Initially, 33 of 36 urine specimens containing greater than 10(5) gram-negative bacteria per ml were detected by LAL assay. The three false-negative LAL tests were the result of urine pH levels below the pH minimum for LAL gelation; neutralization of these urine specimens resulted in positive LAL assays and 100% correlation with culture results. All 36 bacteriuric urine specimens were LAL positive within 15 min, with the majority of assays (86.1%) being positive after only 10 min of incubation at 37 degrees C. These data compared favorably with gelation times of 15 min when 1 X 10(5) to 2 X 10(5) gram-negative bacteria per ml were added to sterile urine. Two urine samples obtained from male patients with culture-proven gonococcal urethritis yielded positive LAL assays. The LAL assay was shown to correctly differentiate 96.2% of urine specimens as containing less than 1...Continue Reading


Nov 1, 1982·Indian Journal of Pediatrics·A Ansari, R Nachum
Sep 1, 1991·Indian Journal of Pediatrics·S Singh

Related Concepts

Enterobacteriaceae Infections
False Positive Reactions
Hydrogen-Ion Concentration
Limulus Test
Pseudomonas Infections

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