Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis

Diagnostic Microbiology and Infectious Disease
Steven Y C TongP Giffard

Abstract

We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform.

References

Oct 6, 2006·Journal of Clinical Microbiology·Flavia HuygensPhilip M Giffard
Jul 6, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·D J WolterR V Goering
May 27, 2008·Experimental and Molecular Pathology·Maria EraliCarl T Wittwer
Apr 28, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S Y C TongP M Giffard

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Citations

Mar 20, 2012·PloS One·Patiyan AnderssonPhilip M Giffard
Jan 31, 2012·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Lenka Ruskova, Vladislav Raclavsky
Oct 27, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Raiane Cardoso ChamonKátia Regina Netto Dos Santos

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