Rapid Detection of Hypervirulent Serovar 4h Listeria monocytogenes by Multiplex PCR

Frontiers in Microbiology
Youwei FengXin'an Jiao

Abstract

Listeria monocytogenes (L. monocytogenes) is a ubiquitous foodborne pathogen that comprises 14 serotypes, of which serovar 4h is a novel serotype recently reported. Serovar 4h L. monocytogenes belonging to hybrid sub-lineage II exhibit hypervirulent features. Conventional biochemical tests and widely used PCR-based serogrouping schemes could not distinguish serovar 4h strains. In this study, we developed a new multiplex PCR assay for rapid detection of serotype 4h L. monocytogenes. Three primer pairs based on the target genes, LMxysn_1095, lmo1083, and smcL, were designed. The multiplex PCR results showed that serovar 4h strains could be specifically identified from all tested strains, including various L. monocytogenes serovars, Listeria spp., and other species. The detection limits of the multiplex PCR were 291 fg/μL for genomic DNA and 5.5 × 106 CFU/mL for bacterial suspension. Furthermore, pork meat artificially contaminated with serovar 4h L. monocytogenes in a concentration of 1.8 × 103-1.8 × 100 CFU/10 g were successfully detected within 10-16 h. These results demonstrate that the multiplex PCR with high specificity and sensitivity is applicable for the rapid detection of L. monocytogenes serotype 4h strains.

References

Jan 1, 1997·Letters in Applied Microbiology·S LoncarevicW Tham
Jul 4, 2001·Clinical Microbiology Reviews·J A Vázquez-BolandJ Kreft
Aug 7, 2004·Journal of Clinical Microbiology·Michel DoumithPaul Martin
Nov 29, 2005·Journal of Microbiological Methods·Robin L T ChurchillJ Christopher Hall
Mar 4, 2006·Microbiology·Angela RobertsMartin Wiedmann
Aug 25, 2007·International Journal of Food Microbiology·Hua YangYaping Sun
Oct 22, 2008·Applied and Environmental Microbiology·Todd J WardJoseph P Bielawski
Nov 8, 2008·International Journal of Food Microbiology·Sara LomonacoTiziana Civera
Oct 9, 2009·Current Topics in Microbiology and Immunology·Sukhadeo B Barbuddhe, Trinad Chakraborty
Mar 27, 2010·Journal of Applied Microbiology·D Rodríguez-LázaroM Hernández
Aug 17, 2010·International Journal of Medical Microbiology : IJMM·Renato H OrsiMartin Wiedmann
Mar 31, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Anne Kvistholm JensenSteen Ethelberg
Nov 1, 2017·Iranian Journal of Basic Medical Sciences·Ali RazeiHosien Aghamollaei
Nov 28, 2017·Nature Reviews. Microbiology·Lilliana Radoshevich, Pascale Cossart
Jul 20, 2018·Journal of Medical Microbiology·Anna DurantiFrancesco Pomilio
Oct 2, 2019·Nature Communications·Yuelan YinXin'an Jiao

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Methods Mentioned

BETA
PCR
accepted
electrophoresis
glycosylation

Software Mentioned

- BLAST
Primer Premier
Primer

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