Rapid detection of intestinal carriage of Klebsiella pneumoniae producing KPC carbapenemase during an outbreak

The Journal of Hospital Infection
T GianiG M Rossolini

Abstract

Two different approaches are described for rapid detection of intestinal carriage of Klebsiella pneumoniae producing KPC-type carbapenemase (KPC-KP), based on PCR amplification of DNA extracts from rectal swabs (K-PCR), and on direct plating of rectal swabs on to MacConkey agar with a meropenem disc and a meropenem plus 3-aminophenylboronic acid disc (direct KPC screening test, DKST). K-PCR and DKST were tested with a total of 101 samples from 65 patients, during an outbreak. Although less sensitive, DKST could detect high-level carriage, which appears to be common among infected and colonised patients, while being very cheap and easy to perform, and requiring only basic facilities.

References

Aug 30, 2008·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·David Calfee, Stephen G Jenkins
Nov 1, 2008·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Gopi PatelDavid P Calfee
Sep 18, 2009·Journal of Clinical Microbiology·Tommaso GianiGian Maria Rossolini
Jan 21, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·V MiriagouR Cantón
Dec 24, 2010·International Journal of Antimicrobial Agents·Theofano PanageaHelen Giamarellou
Sep 9, 2011·Journal of Clinical Microbiology·Antonella AgodiAthanassios Tsakris
Oct 18, 2011·Emerging Infectious Diseases·Patrice NordmannLaurent Poirel

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Citations

Jul 12, 2013·Journal of Clinical Microbiology·Spyros PournarasAthanassios Tsakris
Apr 29, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C TasciniF Menichetti
Mar 15, 2015·Diagnostic Microbiology and Infectious Disease·Alberto AntonelliGian Maria Rossolini
Oct 14, 2014·Bone Marrow Transplantation·C GirmeniaUNKNOWN Gruppo Italiano Trapianto Midollo Osseo GITMO
Oct 28, 2020·Antimicrobial Agents and Chemotherapy·Vincenzo Di PilatoGian Maria Rossolini
Apr 4, 2021·International Journal of Environmental Research and Public Health·Beatrice CasiniGaetano Privitera

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