Rapid detection of Listeria monocytogenes in ham samples using immunomagnetic separation followed by polymerase chain reaction

Journal of Applied Microbiology
J A HudsonR E McCormick

Abstract

To develop a 24-h system for the detection of Listeria monocytogenes in ham. An immunomagnetic separation (IMS) of bacteria directly from ham followed by extraction of DNA and detection using a new multiplex polymerase chain reaction (PCR) was used. The PCR method used one primer pair targeted at the listeriolysin O gene of L. monocytogenes and the other pair for a region of the 23S rRNA genes of Listeria, giving products of 706 and 239 bp, respectively. The combined IMS/PCR was calculated to be capable of detecting as few as 1.1 L. monocytogenes cells g-1 in a 25-g ham sample. The process produced acceptable results, but the IMS step is the main barrier to further improvement of sensitivity. The DNA isolation was the most time-consuming step in the process. A 24-h test for the presence of L. monocytogenes will be useful to the food industry and significantly assist in the timely investigation of outbreaks.

References

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Citations

Mar 19, 2008·Analytical and Bioanalytical Chemistry·S R Nugen, A J Baeumner
Apr 23, 2003·International Journal of Food Microbiology·Patrice GracieuxPhilippe Velge
Nov 27, 2002·Applied and Environmental Microbiology·Luca CocolinGiuseppe Comi
May 15, 2010·Yi chuan = Hereditas·Ling-Wei WuZu-Hong Lu
Jun 24, 2010·American Journal of Infection Control·Ianick Souto MartinsBeatriz Meurer Moreira
Mar 19, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Vanessa de Vasconcelos ByrneRogeria Comastri de Castro Almeida
Dec 17, 2009·Biotechnology Advances·Vijayalakshmi VelusamyCatherine Adley
Jan 1, 2008·International Journal of Food Microbiology·Dongyou Liu
Aug 25, 2007·International Journal of Food Microbiology·Hua YangYaping Sun
Sep 12, 2006·Journal of Microbiological Methods·Alka Mukhopadhyay, Utpal K Mukhopadhyay
Nov 29, 2005·Journal of Microbiological Methods·Robin L T ChurchillJ Christopher Hall
Oct 13, 2005·FEMS Microbiology Reviews·Uta GasanovPhilip M Hansbro
Aug 15, 2013·Journal of Biotechnology·Soo Hwan Suh, Lee-Ann Jaykus
Feb 23, 2008·Advances in Food and Nutrition Research·Arun K Bhunia
Apr 20, 2011·Revista Da Sociedade Brasileira De Medicina Tropical·Cristhiane Moura Falavina dos ReisErnesto Hofer
Oct 12, 2010·Critical Reviews in Microbiology·Hari P Dwivedi, Lee-Ann Jaykus
Sep 30, 2019·Journal of Dairy Science·M AndrettaL A Nero
Jan 13, 2005·Canadian Journal of Microbiology·Kiev S Gracias, John L McKillip
Feb 18, 2020·Journal of Food Protection·Danilo A L SilvaLuís A Nero
Oct 24, 2003·Journal of Food Protection·Patrick W Benoit, Darrell W Donahue

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