PMID: 9163656May 1, 1997Paper

Rapid detection of multidrug-resistant tuberculosis

The European Respiratory Journal
M GoyalD B Young

Abstract

Transmission of multidrug-resistant strains of Mycobacterium tuberculosis (MDR-TB) presents a serious problem for infection control in hospitals, particularly in the context of co-infection with the human immunodeficiency virus (HIV). We report on the use of molecular genetic tools to allow rapid assessment of samples from patients potentially infected with MDR-TB. Sputum and bronchoalveolar lavage samples were obtained from two HIV-positive patients with suspected tuberculosis, who had previous contact with a known MDR-TB index case. Polymerase chain reaction (PCR) was used directly on clinical samples to amplify genetic loci associated with rifampicin resistance (rpoB), and strain-specific polymorphisms (the direct repeat (DR) region). Drug resistance was determined using a commercially available kit for detection of point mutations in the rpoB gene (Inno-Lipa RifTB; Innogenetics, Belgium), and confirmed by nucleotide sequencing. Strain variation was determined using the spoligotyping method, based on the presence or absence of variable linker sequences within the DR region. In one patient, infection with a MDR strain identical to that of a known index case was demonstrated. A second patient, although positive for M. tubercul...Continue Reading

Citations

Mar 17, 1999·Veterinary Microbiology·P DandapatV M Katoch
Jan 16, 1999·Current Opinion in Biotechnology·D B Young, B D Robertson
Jun 11, 1999·Critical Care Medicine·G Santis, T W Evans
Jan 25, 2002·Annals of the New York Academy of Sciences·P Ormerod
Oct 5, 2001·Clinical Microbiology Reviews·A C FluitF J Schmitz
Dec 10, 2003·Journal of Clinical Microbiology·Holger MaytaUNKNOWN Tuberculosis Working Group in Perú
Sep 22, 2009·Canadian Journal of Microbiology·Patricia Izquierdo CafruneArnaldo Zaha
Jul 11, 2008·PLoS Medicine·Frank G J CobelensUNKNOWN Working Group on MDR-TB of the Stop TB Partnership
Mar 12, 1998·Infectious Disease Clinics of North America·W Z Bradford, C L Daley
Apr 4, 2001·Memórias do Instituto Oswaldo Cruz·S Spindola de MirandaE Drouet
Sep 1, 2005·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·N VezirisV Jarlier

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