Rapid detection of Mycobacterium tuberculosis cells by using microtip-based immunoassay

Analytical and Bioanalytical Chemistry
Woon-Hong YeoKyong-Hoon Lee

Abstract

This paper describes a microtip-based approach of concentrating target analytes for a highly sensitive bioassay. As an example, rapid screening of bacterial whole cells is presented to detect Mycobacterium tuberculosis (MTB), a pathogenic bacterium for human tuberculosis (TB). The concentration and detection is performed with three sequential steps of (1) attracting bacterial whole cells in the vicinity of a microtip by alternating current electroosmotic flow; (2) capturing the cells onto the microtip by capillary action; (3) binding fluorophore-labeled polyclonal antibodies to the cells followed by fluorescence measurement (immunofluorescence). Through this mechanism, MTB cells have been detected to the concentration of 8,000 cells/mL within 10 min. This sensitivity is comparable to that of Ziehl-Neelsen smear microscopy, a common culture-free screening method for diagnosis of TB. For comparison, Escherichia coli O157:H7 cells have also been detected to the concentration of 30,000 cells/mL in the same way.

References

Sep 1, 1992·Journal of Clinical Microbiology·E SadaT Herrera
Aug 1, 1993·Diagnostic Microbiology and Infectious Disease·G F ArajM Abu-Salim
May 12, 2000·American Journal of Respiratory and Critical Care Medicine·E D ChanW E Hahn
Jun 2, 2000·Journal of Clinical Microbiology·S PottumarthyA J Morris
Jun 2, 2000·Journal of Clinical Microbiology·L M Pereira Arias-BoudaA H Kolk
Sep 15, 2000·Journal of Immunological Methods·K P LyashchenkoM L Gennaro
Jun 17, 2004·Biosensors & Bioelectronics·Darren S GrayChristopher S Chen
Dec 2, 2004·Analytical Chemistry·Pak Kin WongChih-Ming Ho
Mar 3, 2005·Biosensors & Bioelectronics·María Díaz-GonzálezAgustín Costa-García
Apr 14, 2005·Nano Letters·Paul E Sheehan, Lloyd J Whitman
Nov 12, 2005·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·V C C ChengK Y Yuen
Apr 29, 1966·Science·H A Pohl, I Hawk

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Citations

Mar 8, 2012·Lab on a Chip·Jong-Hoon KimJae-Hyun Chung
Apr 19, 2013·The Analyst·Dinesh KalyanasundaramJae-Hyun Chung
Mar 26, 2011·Clinical & Developmental Immunology·Lixia ZhouDilan Qin
Jan 30, 2013·Biotechnology Advances·ShuQi WangUtkan Demirci
Jan 19, 2016·Expert Review of Molecular Diagnostics·ShuQi WangUtkan Demirci
Sep 3, 2014·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Taha Roodbar ShojaeiRaheleh Jorfi
Sep 26, 2019·Critical Reviews in Biotechnology·Behrouz GolichenariJavad Behravan
Apr 16, 2013·RSC Advances·Jong-Hoon KimJae-Hyun Chung
Aug 10, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·M PoetschkeG Cuniberti

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