Abstract
The feasibility of using an impedance method for direct detection of oxacillin-resistant Staphylococcus aureus (ORSA) in blood cultures was evaluated. An aliquot (0.1 ml) of the positive blood culture, which showed growth of gram-positive cocci and demonstrated thermonuclease activity, was inoculated into the module well of a Bactometer incubator (bioMerieux Vitek, Hazelwood, Mo.) containing 0.6 ml of Mueller-Hinton agar supplemented with oxacillin (2 microg/ml). The modules were incubated at 37 degrees C, and the change in impedance in each well was continuously monitored by the instrument at 6-min intervals for 24 h. ORSA strains from blood cultures could multiply in the oxacillin-containing medium, and a time point (detection time [DT]) at which an accelerating change of impedance occurred in the medium was obtained, with an average of 5.5 h. The growth of oxacillin-sensitive S. aureus (OSSA) strains was largely inhibited, and no DT was obtained for these strains within an incubation period of 24 h. For 96 positive blood cultures (38 ORSA and 58 OSSA) tested, 36 and 57 were found to be oxacillin resistant and oxacillin sensitive, respectively, by the impedance method. The impedance method had a sensitivity and specificity of...Continue Reading
References
Nov 1, 1992·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M R VisserJ Verhoef
Jun 1, 1989·Journal of Clinical Microbiology·G M TrenholmeS Levin
May 1, 1989·The Journal of Applied Bacteriology·R D Bullock, D Frodsham
Dec 1, 1987·Antimicrobial Agents and Chemotherapy·H F Chambers, C J Hackbarth
Sep 1, 1986·Reviews of Infectious Diseases·J A Washington, D M Ilstrup
Oct 1, 1986·The Journal of Applied Bacteriology·I D Ogden
Aug 29, 1968·The New England Journal of Medicine·F F BarrettM Finland
Jun 1, 1984·Journal of Clinical Microbiology·J M Boyce
Sep 1, 1983·Journal of Clinical Microbiology·B M Madison, V S Baselski
Nov 1, 1983·Journal of Clinical Microbiology·C Thornsberry, L K McDougal
Dec 1, 1982·Journal of Clinical Microbiology·G V Doern, L I Robbie
May 1, 1982·American Journal of Clinical Pathology·C J Shanholtzer, L R Peterson
Jan 1, 1983·Reviews of Infectious Diseases·M P WeinsteinK A Lichtenstein
Sep 1, 1982·Annals of Internal Medicine·R W HaleyJ M Hughes
Apr 1, 1995·Pathology·A McGregorG Gilbert
Jan 1, 1995·Journal of Clinical Microbiology·C L McDonald, K Chapin
Jun 1, 1994·Journal of Dairy Science·H C Chen, T C Chang
Feb 1, 1994·Journal of Clinical Microbiology·C C KnappJ A Washington
Aug 1, 1995·Journal of Food Protection·Tsung C Chang, Su H Huang
Citations
Jan 26, 2002·Journal of Food Protection·Tsung C ChangShiowwen Chen
Oct 27, 2010·PloS One·Boris ZavizionRonald J Rieder
Jan 10, 2013·Annals of Laboratory Medicine·Alex van BelkumWilliam Michael Dunne
Sep 17, 1998·Journal of Clinical Microbiology·A H HuangT C Chang
May 23, 1998·Journal of Clinical Microbiology·N MirR Cantón
Oct 4, 2000·Journal of Clinical Microbiology·T C Chang, A H Huang
Mar 17, 2004·Diagnostic Microbiology and Infectious Disease·Jiunn Jong WuTsung Chain Chang
Mar 4, 2000·Journal of Clinical Microbiology·H C ChangT C Chang