Rapid detection of pigeon herpesvirus, fowl adenovirus and pigeon circovirus in young racing pigeons by multiplex PCR

Journal of Virological Methods
Markus FreickR Raue

Abstract

Infections of young racing pigeons with pigeon herpesvirus (PiHV), fowl adenovirus (FAdV) and pigeon circovirus (PiCV) are reported frequently. The role of these viruses in the pathogenesis of a disease complex called young pigeon disease syndrome (YPDS) is generally accepted. All of these viruses cause inclusion bodies in the liver so liver samples are particularly useful for the detection of infection. Consequently a multiplex polymerase chain reaction (PCR) was developed for the detection of PiHV, FAdV and PiCV in liver samples from racing pigeons. The detection limits were 10(1) genome equivalents for the detection of PiHV and PiCV and 10(3) genome equivalents for FAdV. The absence of PCR inhibitors was shown by the detection of cytochrome B gene as an internal control. No PCR products were amplified from related herpes and circoviruses or negative controls, demonstrating the specificity of the multiplex PCR. The addition of cellular DNA from liver samples or Q-solution to the reaction mix had no influence on its sensitivity. The usefulness of the multiplex PCR was demonstrated by re-investigation of liver samples from young racing pigeons previously tested positive by uniplex PCRs.

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Citations

Mar 23, 2013·BMC Veterinary Research·Grzegorz J WoźniakowskiMarek Houszka
Nov 7, 2015·The Veterinary Journal·Panpan ZhaoMingwei Xing
Mar 25, 2014·Acta Veterinaria Hungarica·Tomasz Stenzel, Daria Pestka
Oct 29, 2014·Archives of Virology·Zhicheng ZhangDingzhen Dai
Sep 13, 2011·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Zhicheng ZhangHongjie Fan
Jan 31, 2017·Avian Pathology : Journal of the W.V.P.A·Yen-Li HuangHung-Yi Wu
Dec 12, 2018·Transboundary and Emerging Diseases·Qing-Ye ZhuangKai-Cheng Wang
Nov 3, 2012·The Veterinary Record·T A StenzelA Koncicki

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