PMID: 6302222Jan 1, 1983Paper

Rapid detection of rotavirus in stool by latex agglutination: comparison with radioimmunoassay and electron microscopy and clinical evaluation of the test

Journal of Medical Virology
O J HaikalaH K Sarkkinen

Abstract

A latex agglutination test (LX) using antisera prepared against Nebraska calf diarrhea virus (NCDV) is described for the detection of rotavirus in stool of children with acute gastroenteritis. The test was compared with electron microscopy (EM) and radioimmunoassay (RIA) with 100 stools positive or negative for rotavirus. Out of 53 stools positive in RIA or EM, 49 were positive in LX and 4 were negative. Two specimens negative in EM and RIA were falsely positive in LX. The method was also tested in two clinical series with 115 stools from 101 children. Altogether 67/115 stools were positive in RIA, and 62/115 in LX. Out of 7 stools with contradictory results, 6 were negative in LX but positive in RIA, and 1 was positive in LX but negative in RIA. The results indicate that the LX is suitable for rapid screening of rotavirus gastroenteritis in clinical practice.

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Citations

Mar 1, 1989·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E KohliA Goudeau
Apr 1, 1984·European Journal of Clinical Microbiology·F MorinetY Pérol
Jan 1, 1989·Pharmacology & Therapeutics·M L LandryG D Hsiung
Mar 1, 1987·Diagnostic Microbiology and Infectious Disease·S M GoyalK A Pomeroy
Dec 1, 1987·Diagnostic Microbiology and Infectious Disease·M J GilchristR L Ward
Dec 1, 1988·Diagnostic Microbiology and Infectious Disease·P H Dennehy, D R Gauntlett
Jan 1, 1989·Clinical Microbiology Reviews·M L Christensen
May 1, 2001·Clinical and Diagnostic Laboratory Immunology·Y Al-YousifS Kapil
Jan 1, 1985·Scandinavian Journal of Infectious Diseases·I JulkunenT Hovi
Jun 1, 1995·Annals of Tropical Paediatrics·M K HendricksC A Hart
Jun 1, 1988·Journal of Clinical Microbiology·E E ThomasM Curran
Sep 1, 1984·Journal of Clinical Microbiology·J H HughesV V Hamparian
Jun 1, 1984·Microbiological Reviews·G Cukor, N R Blacklow
Nov 1, 1988·Journal of Clinical Microbiology·B MarchlewiczJ Lampinen
Nov 1, 1988·Journal of Clinical Microbiology·M CherneskyL Schaefer
Sep 1, 1985·Journal of Clinical Microbiology·M CherneskyD DeLong
Nov 1, 1985·Journal of Clinical Microbiology·C H PaiB Ince
Mar 1, 1989·Journal of Clinical Microbiology·A D Steele

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