Rapid detection of Staphylococcus aureus by a combination of monoclonal antibody-coated latex and capillary electrophoresis

Electrophoresis
Peng GaoJing Tian

Abstract

The rapid detection of pathogenic bacteria is extremely important in biotechnology and clinical diagnosis. CE has been utilized in the field of bacterial analysis for many years, but to some extent, simultaneous separation and identification of certain microbes from complex samples by CE coupled with UV detector is still a challenge. In this paper, we propose a new strategy for rapid separation and identification of Staphylococcus aureus (S. aureus) in bacterial mixtures by means of specific mAb-coated latex coupled with CZE. An appropriate set of conditions that selectively isolated S. aureus from the microorganisms Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae were established. S. aureus could be differentiated from the others by unique peaks in the electropherograms. The validity was also confirmed by LIF with antibodies specific to both the latex and the microbial cells. The LOD is as low as 9.0 x 10(5) colony forming unit/mL. We have also utilized this technology to identify S. aureus in a stool sample coming from a healthy volunteer spiked successfully with S. aureus. This CZE-UV technique can be applied to rapid diagnosis of enteritis caused by S. aureus or other bacterial control-related fields nee...Continue Reading

References

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Citations

Apr 4, 2007·Journal of Chromatography. a·Karine FaureJean-Louis Rocca
Jun 26, 2008·Electrophoresis·Vratislav Kostal, Edgar A Arriaga
Oct 18, 2007·Electrophoresis·Virginia García-Cañas, Alejandro Cifuentes
Oct 24, 2008·Electrophoresis·Bogusław Buszewski, Ewa Kłodzińska
Jul 2, 2010·Electrophoresis·Gi Won ShinGyoo Yeol Jung
Dec 18, 2013·Electrophoresis·Ewelina Dziubakiewicz, Bogusław Buszewski
Nov 9, 2017·Molecular Medicine Reports·Ling WeiYong-Zhi Lun
Jan 7, 2009·Analytical Chemistry·Ewa Kłodzińska, Bogusław Buszewski

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