Rapid Detection of the Three Celiac Disease Risk Genotypes HLA-DQ2.2, HLA-DQ2.5, and HLA-DQ8 by Multiplex Ligation-Dependent Probe Amplification

Genetic Testing and Molecular Biomarkers
Raymon VijzelaarWouter Kortlandt

Abstract

Genotyping of HLA-DQ2.2, HLA-DQ2.5, and HLA-DQ8 is important in celiac disease (CD). The absence of these three genotypes has a strong negative predictive value. We designed multiplex ligation-dependent probe amplification (MLPA) for the combined detection of HLA-DQ2.2, HLA-DQ2.5, and HLA-DQ8. The MLPA probe mix was validated against a set of 59 samples characterized by conventional techniques. The MLPA assay genotyped all 59 samples correctly when compared to the results obtained by PCR-SSCP/HD or PCR-SSO and PCR-SSP. The MLPA assay provides a reliable single-reaction analysis of the CD risk genotypes HLA-DQ2.2, HLA-DQ2.5, and HLA-DQ8 allowing for stratification or exclusion of disease risk.

References

Nov 26, 2008·Human Immunology·Francesca MegiorniMaria Cristina Mazzilli
Dec 27, 2011·Journal of Pediatric Gastroenterology and Nutrition·S HusbyUNKNOWN European Society for Pediatric Gastroenterology, Hepatology, and Nutrition
Oct 12, 2012·Journal of Biomedical Science·Francesca Megiorni, Antonio Pizzuti
Oct 23, 2012·Journal of Pediatric Gastroenterology and Nutrition·Amani MubarakRoderick Houwen
Dec 21, 2012·The New England Journal of Medicine·Alessio Fasano, Carlo Catassi
Jan 15, 2013·Clinical Chemistry and Laboratory Medicine : CCLM·Ellen M van BeekWouter Kortlandt
Jun 12, 2014·Gut·Jonas F LudvigssonUNKNOWN British Society of Gastroenterology
Sep 23, 2014·Critical Reviews in Clinical Laboratory Sciences·Vilte E BarakauskasMathew P Estey
Jun 18, 2015·Scandinavian Journal of Clinical and Laboratory Investigation·Flemming LundGyörgy Sölétormos

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Citations

May 9, 2019·Genetic Testing and Molecular Biomarkers·Maxine D RouvroyeHetty J Bontkes

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Methods Mentioned

BETA
genotyping
PCR
electrophoresis
Assay

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