Rapid detection of viable yeasts and bacteria in wine by flow cytometry

Journal of Microbiological Methods
P MalacrinòF Dellaglio

Abstract

The potential of using flow cytometry (FCM) in combination with fluorescent dyes for rapidly estimating counts of yeasts and malolactic bacteria in laboratory media and wines was examined. In general, there was a good correlation (regression coefficient, 0.94) between viable counts of yeasts determined by FCM and by standard plate assay. The FCM detection limit of yeasts in YPDE medium and in Pinot noir must was 10(3) cells/ml. The lowest bacterial concentration detected by FCM was 10(4) cells/ml. When yeast and malolactic bacteria populations were simultaneously analysed in wine by FCM without any previous sample treatment, difficulties were encountered in the count of bacterial cells due to their size, which is similar to natural debries present in wine. However, after the optimisation of the sample preparation, the technique appeared promising in determining the presence of such microorganisms in wine with one single measurement. Because it is rapid and easy to use, flow cytometry can be considered a useful method for microbiological quality control in wineries and for the investigation of the growth dynamics of microorganisms in wine.

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Citations

Jun 27, 2009·Journal of Industrial Microbiology & Biotechnology·Jaume Comas-Riu, Núria Rius
Aug 9, 2011·Journal of Industrial Microbiology & Biotechnology·Melissa L Ivey, Trevor G Phister
Aug 7, 2012·Journal of Industrial Microbiology & Biotechnology·Leo L ChanAlnoor Pirani
Oct 20, 2012·Journal of Industrial Microbiology & Biotechnology·Mohammad SalmaHervé Alexandre
May 9, 2002·Journal of Microbiological Methods·Karen VermisHans J Nelis
Jun 5, 2003·Journal of Microbiological Methods·J R Bradner, K M H Nevalainen
Oct 6, 2006·Applied and Environmental Microbiology·Mónica HerreroMario Díaz
Jan 3, 2013·Journal of Applied Microbiology·M Bouix, S Ghorbal
Feb 1, 2006·Meat Science·L A López TomásG García de Fernando
Mar 24, 2006·Journal of Applied Microbiology·D ChaneyR Thornton
Jul 17, 2012·Journal of Microbiological Methods·Julie MonthéardSandrine Alfenore
Jul 20, 2005·International Journal of Food Microbiology·María Belén MayoralTeresa García
Nov 22, 2005·Critical Reviews in Biotechnology·R E Madrid, C J Felice
Sep 19, 2001·Yeast
May 10, 2016·Journal of Applied Microbiology·N A ReisN S Evangelista-Barreto
Nov 1, 2017·Frontiers in Microbiology·Bahareh BagheriMathabatha E Setati
Nov 4, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Louise BartleJames S Paterson
May 3, 2002·Analytical Chemistry·Matthew M FerrisKathy L Rowlen
Sep 12, 2020·Food Microbiology·Adriana Nunes de LimaJosé António Couto

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