Rapid detection of Vibrio cholerae O139 Bengal from stool specimens by PCR.

Journal of Clinical Microbiology
M John AlbertAndrej Weintraub

Abstract

In a previous study using pure bacterial cultures in a PCR assay, a primer pair corresponding to a unique chromosomal region of Vibrio cholerae O139 Bengal generated an amplicon from only V. cholerae O139 Bengal. PCR with the same primer pair was used to screen 180 diarrheal stool specimens. All the 67 V. cholerae O139 culture-positive stool specimens were positive by PCR, and the remaining specimens, which contained either other recognized enteric pathogens or no pathogens, were all negative by PCR.

References

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Citations

Jun 28, 2001·Clinical and Diagnostic Laboratory Immunology·A Martínez-GoveaA Flisser
Jul 17, 2003·Current Gastroenterology Reports·James V Lawler, Mark R Wallace
Sep 8, 2006·Applied and Environmental Microbiology·Aneta J Gubala, David F Proll
Sep 20, 2007·The Journal of General and Applied Microbiology·Chariya ChomvarinChulapan Engchanil
Aug 10, 2011·Critical Reviews in Microbiology·Chandrika Murugaiah
Dec 25, 2010·Journal of Medical Microbiology·Ang Lim ChuaPattabhiraman Lalitha
Oct 23, 2013·The Journal of Infectious Diseases·Karen H KeddyAnthony M Smith
Apr 23, 2003·Microbes and Infection·Thandavarayan RamamurthyGopinath Balakrish Nair
Jun 12, 1999·Journal of Clinical Microbiology·W A GramleyS M Powell

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