PMID: 11311357Apr 20, 2001Paper

Rapid determination of adenoviral vector titers by quantitative real-time PCR

Journal of Virological Methods
L MaH H van Es

Abstract

Replication defective adenoviruses have been used as vectors in a variety of settings including gene transfer, gene manipulation, and functionality studies. A quantitative real-time PCR-based assay is described for rapid determination of physical titers of recombinant adenovirus vectors. This method is based on amplification of a 77 bp fragment located near the left end of the adenovirus type 5 genome. Evaluation of this method demonstrated that it is simple, sensitive and reproducible, and has a dynamic range of quantitation over 5 logs. This assay is applicable to purified adenovirus as well as vectors prepared by simple cell lysis procedure, requiring only a small amount of starting material. The simplicity and short turn-around time of this assay should facilitate rapid titer determination for a large collection of adenoviral vectors.

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Citations

Sep 1, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Ivan TancevskiAndreas Ritsch
Feb 1, 2003·Veterinary Parasitology·Wooseog JeongSang Gi Paik
May 15, 2002·Diagnostic Microbiology and Infectious Disease·Huo-Shu H HoungLeonard N Binn
Aug 30, 2008·Gene Therapy·L KangasniemiA Hemminki
Sep 17, 2003·Genome Research·Gert-Jan ArtsHelmuth van Es
Mar 19, 2009·World Journal of Gastroenterology : WJG·Michael-A van GeerPiter J Bosma
Jun 13, 2009·World Journal of Gastroenterology : WJG·Michael A van GeerPiter J Bosma
Sep 16, 2009·Journal of Virological Methods·María Mercedes SeguraMiguel Chillón
Dec 14, 2011·Reviews in Medical Virology·Tina Ganzenmueller, Albert Heim
May 14, 2004·Journal of Biomolecular Screening·Kaumudi M BhawePeter M Flanagan
Dec 24, 2005·Expert Review of Vaccines·Jose A LebronBrian J Ledwith
Jan 7, 2005·Journal of Biomolecular Screening·Robert L YauchDouglas O Clary
Mar 15, 2006·International Journal of Biological Macromolecules·Ivan TancevskiAndreas Ritsch
Sep 5, 2008·Journal of Lipid Research·Joerg F RippmannJuergen Prestle
Sep 29, 2007·The Journal of Gene Medicine·Maarten ter HorstPeter Sillevis Smitt
Oct 2, 2002·Nature Biotechnology·Frits MichielsJoost Donker
Aug 3, 2007·Expert Review of Vaccines·Jayanthi J WolfFubao Wang

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