Rapid determination of N,N-diethyl-m-toluamide and permethrin in human plasma by gas chromatography-mass spectrometry and pyridostigmine bromide by high-performance liquid chromatography

Journal of Analytical Toxicology
Svetlana A CherstniakovaLouis R Cantilena

Abstract

A rapid and highly sensitive gas chromatography-mass spectrometry (GC-MS) method for simultaneous determination of N,N-diethyl-m-toluamide (DEET) and permethrin with (2)H(10)-phenanthrene (98 atom %) as an internal standard and a separate external standard high-performance liquid chromatography (HPLC) method for pyridostigmine bromide (PB) determination in human plasma were developed and validated. The GC-MS method for DEET and permethrin quantification utilizes a one-step extraction with tert-butylmethylether. The HPLC method for PB quantification involves a solid-phase extraction and UV detection. The range of the analytical method for DEET and permethrin was 1 ng/mL to 100 ng/mL and for PB was 5 ng/mL to 100 ng/mL. Recovery from plasma proved to be more than 80%. The intraday precision ranged from 1.3% to 8% for DEET, from 2.1% to 11.4% for permethrin, and from 3.0% to 4.8% for PB. The interday precision was 3% for DEET, ranged from 5% to 9% for permethrin, and from 5% to 9% for PB. The accuracy for the limit of quantification was 92% +/- 8% relative standard deviation (RSD) for DEET, 112% +/- 11% RSD for permethrin, and 109% +/- 5% RSD for PB. All 3 compounds were stable in human plasma at -80 degrees C for at least 12 mont...Continue Reading

Citations

Jul 9, 2014·Journal of Analytical Toxicology·Devin WilesMarcel Casavant
Jul 14, 2010·Pest Management Science·Gretchen PaluchJoel Coats
May 21, 2013·Pharmaceutical Development and Technology·Ana Carolina Moreira SouzaNeila Márcia Silva Barcellos
Nov 15, 2006·Journal of Mass Spectrometry : JMS
Nov 29, 2017·Journal of Analytical Methods in Chemistry·Jadranka MilikićBiljana Šljukić
May 18, 2007·Analytical Chemistry·T A BrettellJ R Almirall

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