Rapid determination of nonaromatic organic acids in honey by capillary zone electrophoresis with direct ultraviolet detection

Journal of Agricultural and Food Chemistry
Inés MatoM Teresa Sancho

Abstract

A rapid capillary zone electrophoresis (CZE) method with direct ultraviolet (UV) detection has been set up and developed to determine the most important nonaromatic organic acids in honey with a really simple treatment of the sample. The determination of oxalic, formic, malic, succinic, pyruvic, acetic, lactic, citric, and gluconic acids has been carried out in 4 min. The electrolyte composition was phosphate as the carrier buffer (7.5 mM NaH(2)PO(4) and 2.5 mM Na(2)HPO(4)), 2.5 mM tetradecyltrimethylammonium hydroxide (TTAOH) as electroosmotic flow modifier, and 0.24 mM CaCl(2) as selectivity modifier, with the pH adjusted at 6.40 constant value. The running voltage was -25 kV at a thermostated temperature of 25 degrees C. The injections were performed in hydrodynamic mode (30 s), and the detection mode was UV direct at 185 nm. Validation parameters of the method as detection and quantification limits, linearity, precision (repeatability and reproducibility), and recovery were also studied. The advantages related to the technique such as simplicity, short analysis times, and low consumption of chemicals as well as the good validation parameters obtained for this method permit it to be considered as adequate for routine analysi...Continue Reading

References

Feb 7, 2001·Journal of Agricultural and Food Chemistry·I MatoM T Sancho
Jul 25, 2000·Journal of Chromatography. a·M J del NozalM Higes
May 16, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Silvia Suárez-LuqueJesús Simal-Lozano
Jun 22, 2002·Journal of Chromatography. a·Silvia Suárez-LuqueM Teresa Sancho
Aug 31, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Suwanna VoraratYodporn Podokmai
Jul 15, 2003·Electrophoresis·Luis Saavedra, Coral Barbas
Jul 1, 1960·Archives of Biochemistry and Biophysics·E E STINSONJ W WHITE
Dec 16, 2003·Journal of Food Protection·Inés MatoM Teresa Sancho
Apr 7, 2004·Journal of Chromatography. a·Verónica Galli, Coral Barbas

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Citations

Jul 28, 2012·Journal of Agricultural and Food Chemistry·Graţia I DezmireanSilvio Erler
Nov 18, 2008·Journal of Medicinal Food·Livia Persano OddoPatricia Vit
Nov 26, 2015·Food Chemistry·Priscila Missio da SilvaRoseane Fett
Apr 14, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marco CiuluGavino Sanna
May 2, 2015·PloS One·Christos NoutsosDoreen H Ware
Sep 7, 2007·Electrophoresis·Christian W Klampfl
Mar 13, 2012·Phytochemical Analysis : PCA·Fernando Antonio Simas VazMarcone Augusto Leal de Oliveira
Sep 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Danila CianciosiMaurizio Battino
Nov 3, 2020·Food Chemistry·Siluana Katia Tischer SeraglioAna Carolina Oliveira Costa
Sep 1, 2017·Comprehensive Reviews in Food Science and Food Safety·Sónia SoaresIsabel Mafra
May 17, 2021·Food Research International·Siluana Katia Tischer SeraglioAna Carolina Oliveira Costa
Mar 12, 2015·Journal of Agricultural and Food Chemistry·Elisabetta SchievanoLuciano Navarini
Mar 20, 2019·Food Research International·Siluana Katia Tischer SeraglioAna Carolina Oliveira Costa
Dec 7, 2006·Journal of Agricultural and Food Chemistry·Silvia Suarez-LuqueM Teresa Sancho

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