Rapid determination of rifaximin in rat serum and urine by direct injection on to a shielded hydrophobic stationary phase by HPLC

Biomedical Chromatography : BMC
R Nageswara RaoSachin B Agawane

Abstract

A simple and rapid reversed-phase HPLC method for determination of rifaximin in rat serum and urine was developed. Separation of rifaximin from biological matrix was achieved by direct injection of rat serum and urine onto a restricted-access medium, Supelco LC-Hisep, a shielded hydrophobic stationary phase, using acetonitrile:water:acetic acid (18:82:0.1 v/v/v) as a mobile phase. The linear range was 0.10-20 microg/mL (r(2 )> 0.999, n = 6), intraday and interday variation was <6.10%. The limits of detection and quantification were 0.03 (signal-to-noise ratio >3) and 0.10 microg/mL (signal-to-noise ratio >10), respectively. The method was successfully applied to pharmacokinetic studies of rifaximin after an oral administration to rats.

References

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Citations

May 16, 2009·Biomedical Chromatography : BMC·R Nageswara RaoDhananjay D Shinde
Jul 20, 2016·Scientifica·Ana Carolina Kogawa, Hérida Regina Nunes Salgado
Mar 31, 2019·Journal of Chromatographic Science·Ana Carolina KogawaHérida Regina Nunes Salgado
Jul 4, 2012·Journal of Separation Science·Ramisetti Nageswara RaoAlamanda Vara Prasada Rao
Feb 3, 2011·Biomedical Chromatography : BMC·R Nageswara RaoPawan K Maurya
Mar 28, 2018·Critical Reviews in Analytical Chemistry·Ana Carolina Kogawa, Hérida Regina Nunes Salgado
May 5, 2021·Journal of Chromatographic Science·Ana Carolina KogawaHérida Regina Nunes Salgado

Related Concepts

Xifaxan
Microbicides
High Pressure Liquid Chromatography Procedure
Rifamycins
Rats, Wistar
Hydrophobicity
Liquid Chromatography
Urine
Acetonitrile
Rifaximin

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