Rapid determination of rifaximin in rat serum and urine by direct injection on to a shielded hydrophobic stationary phase by HPLC

Biomedical Chromatography : BMC
R Nageswara RaoSachin B Agawane


A simple and rapid reversed-phase HPLC method for determination of rifaximin in rat serum and urine was developed. Separation of rifaximin from biological matrix was achieved by direct injection of rat serum and urine onto a restricted-access medium, Supelco LC-Hisep, a shielded hydrophobic stationary phase, using acetonitrile:water:acetic acid (18:82:0.1 v/v/v) as a mobile phase. The linear range was 0.10-20 microg/mL (r(2 )> 0.999, n = 6), intraday and interday variation was <6.10%. The limits of detection and quantification were 0.03 (signal-to-noise ratio >3) and 0.10 microg/mL (signal-to-noise ratio >10), respectively. The method was successfully applied to pharmacokinetic studies of rifaximin after an oral administration to rats.


Oct 12, 1990·Journal of Chromatography·M J Koenigbauer
Jan 1, 1994·Biomedical Chromatography : BMC·A M AndeejaniH Y Aboul-Enein
Sep 22, 1993·Journal of Chromatography·P Campíns-FalcóA Sevillano-Cabeza
Jun 26, 1999·Alimentary Pharmacology & Therapeutics·P GionchettiM Campieri
May 15, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jun MaRong Wang
Jun 18, 2003·The American Journal of Gastroenterology·Robert SteffenHerbert L DuPont
Jan 26, 2005·The Journal of Infection·David B Huang, Herbert L DuPont
Jan 20, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Javier A Adachi, Herbert L DuPont
Dec 23, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xianhua ZhangSuodi Zhai


May 16, 2009·Biomedical Chromatography : BMC·R Nageswara RaoDhananjay D Shinde
Jul 20, 2016·Scientifica·Ana Carolina Kogawa, Hérida Regina Nunes Salgado
Mar 31, 2019·Journal of Chromatographic Science·Ana Carolina KogawaHérida Regina Nunes Salgado
Jul 4, 2012·Journal of Separation Science·Ramisetti Nageswara RaoAlamanda Vara Prasada Rao
Feb 3, 2011·Biomedical Chromatography : BMC·R Nageswara RaoPawan K Maurya
Mar 28, 2018·Critical Reviews in Analytical Chemistry·Ana Carolina Kogawa, Hérida Regina Nunes Salgado
May 5, 2021·Journal of Chromatographic Science·Ana Carolina KogawaHérida Regina Nunes Salgado

Related Concepts

High Pressure Liquid Chromatography Procedure
Rats, Wistar
Liquid Chromatography

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Archaeal RNA Polymerase

Archaeal RNA polymerases are most similar to eukaryotic RNA polymerase II but require the support of only two archaeal general transcription factors, TBP (TATA-box binding protein) and TFB (archaeal homologue of the eukaryotic general transcription factor TFIIB) to initiate basal transcription. Here is the latest research on archaeal RNA polymerases.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.