Rapid determination of venlafaxine and O-desmethylvenlafaxine in human plasma by high-performance liquid chromatography with fluorimetric detection

Journal of Chromatography. B, Biomedical Sciences and Applications
R L VuC Reist

Abstract

A rapid and sensitive high-performance liquid chromatographic technique for simultaneous measurement of plasma venlafaxine (VEN) and its active metabolite O-desmethylvenlafaxine (ODV) is described. The process begins with the extraction of VEN and ODV, with maprotiline (MAP) as internal standard, from human plasma into an intermediate organic mixture of hexane-isoamyl alcohol and finally into an aqueous solution of 0.05% phosphoric acid. Isocratic separation of VEN, ODV and MAP is carried out by utilizing a reversed-phase butyl-bonded column (C4/E) with mobile phase consisting of acetonitrile and 40 mM phosphate buffer, pH 6.8 (50:50, v/v). Detection of VEN, ODV and MAP is done by mean of fluorimetry with excitation and emission wavelengths set at 276 and 598 nm, respectively. As low as 1.0 ng/ml VEN is detectable; while the limit of detection for ODV is 5 ng/ml. C.V. (%) of intra-day samples for both VEN and ODV are less than 10% at three concentrations tested (10.0, 50.0, 100.0 ng/ml). Similarly, over the same nominal concentrations, the precision of inter-day (5 days) samples also results in C.V. (%) smaller than 10% for both compounds, except for ODV measured at 10 ng/ml (C.V.<15%). Approximately, 100% VEN can be extracted ...Continue Reading

Citations

Jun 27, 2002·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Sapna N Makhija, Pradeep R Vavia
Mar 29, 2001·Journal of Clinical Psychopharmacology·E LessardJ Turgeon
Jun 20, 2002·Pharmacology & Toxicology·Lars BrachtendorfUwe Fuhr
Dec 29, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Paraskevas D TzanavarasDemetrius G Themelis
Aug 5, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiang Yang QinAi Dong Wen
Jun 7, 2002·Journal of Pharmaceutical and Biomedical Analysis·Sapna N Makhija, Pradeep R Vavia

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