PMID: 41850Nov 1, 1979

Rapid diagnosis of anaerobic infections by gas-liquid chromatography

Journal of Clinical Pathology
S LadasA Arseni

Abstract

It was postulated that the short chain fatty acids (SCFA) produced by anaerobic bacteria might serve as microbial markers in purulent material. Eighteen pus specimens from various sources were analysed by gas-liquid chromatography (GLC), and the SCFA detected were compared with the microorganisms isolated by conventional methods. It was found that the detection of propionic, isobutyric, butyric, or isovaleric acids by direct GLC of pus specimens is strong evidence for anaerobic infection but not specific for Bacteroides fragilis. It was also shown that the presence of succinic acid in pus specimens does not necessarily indicate infection by anaerobes. It can be concluded that direct GLC of purulent material provides a rapid and reliable presumptive method for the differentiation between anaerobic and aerobic infections.

References

May 1, 1976·Journal of Clinical Pathology·K D PhillipsA T Willis
Feb 1, 1976·The Journal of Clinical Investigation·S L GorbachA B Onderdonk
May 23, 1974·The New England Journal of Medicine·S L Gorbach, J G Bartlett
Mar 31, 1933·The Journal of Experimental Medicine·R C Lancefield

Citations

Dec 1, 1982·European Journal of Clinical Microbiology·W W NicholsK Nicholls
Jul 15, 2005·Journal of Chromatography. a·Martina PetschErnst Kenndler
Apr 10, 2003·Biochemical and Biophysical Research Communications·Niclas E NilssonBjörn Olde
Jul 1, 1982·Journal of Clinical Pathology·B WattA Mitchell
Dec 25, 2008·Cell Biochemistry and Function·M A R VinoloRui Curi
Jan 1, 1997·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·R NiedermanS Kashket
Jan 1, 1981·Critical Reviews in Clinical Laboratory Sciences·D C EdmanJ B Brooks
Dec 22, 2011·Endocrinology·Chiara VoltoliniHenry N Jabbour

Related Concepts

Bacterial Infections
Bacteroides fragilis
Prevotella melaninogenica
Chromatography, Gas-Liquid
Saturated Fat
Genus Fusobacterium
Microbiological Techniques
Pus

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