Rapid diagnosis of Argentine hemorrhagic fever by reverse transcriptase PCR-based assay.

Journal of Clinical Microbiology
M E LozanoVictor Romanowski

Abstract

Argentine hemorrhagic fever (AHF) is an endemo-epidemic disease caused by Junín virus. This report demonstrates that a reverse transcriptase (RT) PCR-based assay developed in our laboratory to detect Junín virus in whole blood samples is sensitive and specific. The experiments were conducted in a double-blinded manner using 94 clinical samples collected in the area in which AHF is endemic. The RT-PCR-based assay was compared with traditional methodologies, including enzyme-linked immunosorbent assay, plaque neutralization tests, and occasionally viral isolation. The calculated parameters for RT-PCR diagnosis, with seroconversion as the "gold standard," were 98% sensitivity and 76% specificity. It is noteworthy that 94% of the patients with putative false-positive results (RT-PCR positive and no seroconversion detected) exhibited febrile syndromes of undefined etiology. These results could be interpreted to mean that most of those patients with febrile syndromes were actually infected with Junín virus but did not develop a detectable immune response. Furthermore, 8 laboratory-fabricated samples and 25 blood samples of patients outside the area in which AHF is endemic tested in a similar way were disclosed correctly (100% match)....Continue Reading

References

Jul 1, 1989·Journal of Clinical Microbiology·D R MoserJ E Donelson
Jul 1, 1985·The Journal of Infectious Diseases·K T McKeeC J Peters
Jan 1, 1986·Medical Microbiology and Immunology·J MaizteguiA Briggiler
Feb 1, 1973·Proceedings of the Society for Experimental Biology and Medicine·C J PetersK M Johnson
Mar 1, 1969·Proceedings of the Society for Experimental Biology and Medicine·P A WebbR B Mackenzie
Sep 1, 1993·Trends in Genetics : TIG·M E LozanoV Romanowski
Nov 1, 1993·Journal of Clinical Microbiology·P G ReddyW Heneine

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Citations

Dec 2, 2008·Journal of Medical Virology·Agustín E UreVíctor Romanowski
Oct 31, 2017·Microbes and Infection·Bjoern Meyer, Allison Groseth
Mar 5, 2003·Antiviral Research·Rémi N Charrel, Xavier de Lamballerie
Mar 5, 2003·Antiviral Research·Christian DrostenStephan Günther
Nov 1, 1996·Journal of Clinical Microbiology·R M WadowskyG D Ehrlich
Oct 10, 2006·Pediatric Clinics of North America·Jiang FanKelly J Henrickson

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Argentine Hemorrhagic Fever

Argentine hemorrhagic fever (AHF) is an endemo-epidemic disease caused by junín virus (JUNV), a member of the arenaviridae family. Discover the latest research on AHF here.