Abstract
Argentine hemorrhagic fever (AHF) is an endemo-epidemic disease caused by Junín virus. This report demonstrates that a reverse transcriptase (RT) PCR-based assay developed in our laboratory to detect Junín virus in whole blood samples is sensitive and specific. The experiments were conducted in a double-blinded manner using 94 clinical samples collected in the area in which AHF is endemic. The RT-PCR-based assay was compared with traditional methodologies, including enzyme-linked immunosorbent assay, plaque neutralization tests, and occasionally viral isolation. The calculated parameters for RT-PCR diagnosis, with seroconversion as the "gold standard," were 98% sensitivity and 76% specificity. It is noteworthy that 94% of the patients with putative false-positive results (RT-PCR positive and no seroconversion detected) exhibited febrile syndromes of undefined etiology. These results could be interpreted to mean that most of those patients with febrile syndromes were actually infected with Junín virus but did not develop a detectable immune response. Furthermore, 8 laboratory-fabricated samples and 25 blood samples of patients outside the area in which AHF is endemic tested in a similar way were disclosed correctly (100% match)....Continue Reading
References
Dec 8, 1979·Lancet·J I MaizteguiA J de Damilano
Apr 1, 1992·Journal of Clinical Microbiology·Y LaziziJ Pillot
Sep 1, 1991·The Journal of General Virology·P D GhiringhelliV Romanowski
Jan 1, 1990·Virology·J CristinaA Portela
Jul 1, 1989·Journal of Clinical Microbiology·D R MoserJ E Donelson
Feb 1, 1989·Journal of Clinical Microbiology·D M Olive
Jun 22, 1989·The New England Journal of Medicine·M F RogersA Kaul
Aug 1, 1989·Journal of Clinical Microbiology·J L BurgJ C Boothroyd
Dec 1, 1988·The Journal of Infectious Diseases·G J DemmlerR A May
Jul 1, 1985·The Journal of Infectious Diseases·K T McKeeC J Peters
Jan 1, 1986·Intervirology·A M AmbrosioJ I Maiztegui
Jan 1, 1986·Medical Microbiology and Immunology·J MaizteguiA Briggiler
Jan 15, 1988·Science·C Y OuG Schochetman
Feb 1, 1973·Proceedings of the Society for Experimental Biology and Medicine·C J PetersK M Johnson
Mar 1, 1969·Proceedings of the Society for Experimental Biology and Medicine·P A WebbR B Mackenzie
Jan 1, 1993·Microbiology and Immunology·A SatoT Kunitomo
Sep 1, 1993·Trends in Genetics : TIG·M E LozanoV Romanowski
Nov 1, 1993·Journal of Clinical Microbiology·P G ReddyW Heneine
Nov 1, 1993·Journal of Clinical Microbiology·T RamamurthyY Uesaka
Aug 26, 1993·Journal of Immunological Methods·A HopertH G Drexler
Jan 1, 1993·Virus Research·M E LozanoO Grau
Jan 1, 1993·Journal of Clinical Microbiology·R M ShawarJ E Clarridge
Citations
Jun 8, 2012·Critical Reviews in Microbiology·Marco GoeijenbierEric Van Gorp
Sep 3, 2003·Biological & Pharmaceutical Bulletin·Takashi KitaharaHitoshi Sasaki
Jun 26, 2009·Clinical and Vaccine Immunology : CVI·Mina NakauchiShigeru Morikawa
Dec 2, 2008·Journal of Medical Virology·Agustín E UreVíctor Romanowski
Oct 31, 2017·Microbes and Infection·Bjoern Meyer, Allison Groseth
Mar 5, 2003·Antiviral Research·Rémi N Charrel, Xavier de Lamballerie
Mar 5, 2003·Antiviral Research·Christian DrostenStephan Günther
Nov 1, 1996·Journal of Clinical Microbiology·R M WadowskyG D Ehrlich
Oct 10, 2006·Pediatric Clinics of North America·Jiang FanKelly J Henrickson