PMID: 2509229Oct 1, 1989

Rapid diagnosis of bacterial meningitis by an enzyme immunoassay of cerebrospinal fluid

Epidemiology and Infection
M A SalihP Olcen

Abstract

A total of 250 cerebrospinal fluid (CSF) specimens were analyzed using a rapid enzyme immunoassay (Pharmacia Meningitis EIA-Test) (EIA) for the detection of antigens of Haemophilus influenzae type b, Neisseria meningitidis (serogroups A,B,C) and Streptococcus pneumoniae (25 selected types). The test is performed in less than 1 h and read by the naked eye. EIA and coagglutination (CoA) were compared with a constructed reference that comprised samples which were either positive by culture and/or on direct microscopy (DM), or in which there were positive results with both EIA and CoA for the bacteria covered by the assays. Using this reference for CSF samples assayed in a period between two meningococcal meningitis epidemics, the sensitivity was 0.86 for EIA and 0.69 for CoA, the specificity 0.95 (EIA) and 0.97 (CoA), the predictive value for a positive result 0.81 (EIA) and 0.87 (CoA) and, the predictive value for a negative result 0.96 (EIA) and 0.93 (CoA). Antibiotics had been given to 54% of the patients before admission. All of the 56 samples that were positive in any of the tests taken during an epidemic of group A meningococcal disease were detected by EIA; CoA was negative in 45% and culture/DM was negative in 32%. Sequent...Continue Reading

References

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Citations

Jun 1, 1994·Journal of Epidemiology and Community Health·H L Ford, J P Wright
Sep 16, 2008·The Journal of Emergency Medicine·Wasiulla RafiSursarla Krishna Shankar
Jan 1, 1992·Scandinavian Journal of Infectious Diseases. Supplementum·M Glimåker
Jan 1, 1990·Scandinavian Journal of Infectious Diseases·M A SalihP Olcen
Feb 4, 2005·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Muhamed-Kheir Taha, P Olcen
Jan 1, 1995·Scandinavian Journal of Infectious Diseases·P OlcenP Rådström
Jan 1, 1992·Annals of Tropical Paediatrics·E J MackieS N Mustafa-Kutana
Jan 1, 1991·Scandinavian Journal of Infectious Diseases·M A SalihP Olcen
Dec 1, 1995·Annals of Tropical Paediatrics·M A SalihP Olcen
Jan 1, 1995·Tropical Doctor·J P Wright, H L Ford
Nov 4, 2009·Scandinavian Journal of Infectious Diseases·P Olcen, Hans Fredlund

Related Concepts

Antigens, Bacterial
Disease Outbreaks
Haemophilus influenzae
Peroxidase-Antiperoxidase Complex Technique
Pachymeningitis
Meningitis, Haemophilus Parainfluenzae
Meningitis, Meningococcal, Serogroup Y
Meningitis, Pneumococcal, Penicillin-Resistant
Neisseria meningitidis
Streptococcus pneumoniae

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