Abstract
Rapid identification of pathogens in patients with bacterial pneumonia is important for optimal antimicrobial therapy. Coagglutination was compared with counterimmunoelectrophoresis (CIE) for sensitivity and specificity in the detection of Streptococcus pneumoniae, Hemophilus influenzae, Klebsiella pneumoniae, and Pseudomonas aeruginosa antigens in sputum specimens of 101 patients with community-acquired pneumonia. Coagglutination detected the appropriate bacterial antigen in 16 of 17 (94%) definite etiology patients and CIE detected 11 (64%). In 17 probable etiology patients, bacterial antigens were detected by coagglutination in 15 (88%) and by CIE in 7 (41%). Only 1 pathogen was falsely identified in the 19 culture-negative control patients, indicating a high degree of specificity for both tests. Coagglutination was more sensitive than CIE (p less than 0.05) or sputum stained with Gram's stain (p less than 0.05) in correctly identifying the putative pathogen in sputum. Our results strongly suggest that coagglutination is a useful diagnostic technique for establishing a rapid etiologic diagnosis in community-acquired pneumonia.
Citations
Jan 1, 1988·Medical Microbiology and Immunology·X P ZhangW T Luo
May 15, 1987·Klinische Wochenschrift·H LodeG Ruckdeschel
Feb 1, 1987·European Journal of Clinical Microbiology·S Lenthe-EboaF A Waldvogel
May 1, 1992·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S Wellstood
Dec 1, 1991·Thorax·W G BoersmaG H Koëter
Sep 9, 2010·Acta Biochimica Et Biophysica Sinica·Yuhua ZhouXiaokui Guo
Mar 1, 1984·Pharmacotherapy·R A Gleckman, R M Roth
May 1, 1989·Indian Journal of Pediatrics·M K LalithaM John
Jan 1, 1988·Scandinavian Journal of Infectious Diseases. Supplementum·K Lehtomäki
Jun 1, 1988·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·V AusinaG Prats
Mar 1, 1993·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·W G BoersmaG H Koëter
Aug 13, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Daniel M MusherAnna Wanahita
Jan 30, 2014·Infection & chemotherapy·Joon Young SongMoon H Nahm
Apr 1, 1987·Journal of Clinical Pathology·O BroganK A McCabe
Feb 1, 1992·Journal of Clinical Microbiology·Y HollowayJ A Snijder
Dec 1, 1993·Journal of Clinical Microbiology·Y HollowayW G Boersma
Oct 12, 1999·Clinics in Chest Medicine·S J Skerrett
Sep 1, 1991·The Journal of Infection·M Farrington, D Rubenstein
Nov 11, 2006·Médecine et maladies infectieuses·O Leroy
Aug 1, 1992·Chest·W G BoersmaG H Koëter
Mar 1, 1986·Diagnostic Microbiology and Infectious Disease·M W Rytel, L C Preheim