Rapid diagnosis of duck plagues virus infection by loop-mediated isothermal amplification

Research in Veterinary Science
Jun JiYing Zuo Bee

Abstract

Duck virus enteritis is a serious disease among farmed and free-living ducks (Anatidae) and a constant threat to the commercial duck industry in China. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed to rapidly detect and diagnose duck plague virus (DPV) in both farmed and wild waterfowl, and compared with polymerase chain reaction (PCR) method and real-time PCR method in accuracy, sensitivity and specificity. A set of four specific primers was successfully designed to recognize six distinct genomic sequences of UL6 protein from DPV, including one forward inner primer, one back inner primer and two outer primers. The optimum reaction temperature and time were verified to be 61.5 degrees C and 60 min, respectively. Comparative experiments showed that LAMP assay was a simple, rapid, accurate, sensitive and specific method for detecting DPV, and was superior to PCR assay in sensitivity and specificity for DNA amplification. In addition, challenge tests indicated the newly developed LAMP method was more sensitive for the diagnosis of DPV infection than virus isolation and PCR. LAMP assay would be a good alternative method for on-farm disease diagnosis.

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Citations

Dec 12, 2013·Archives of Virology·Grzegorz Woźniakowski, Elzbieta Samorek-Salamonowicz
May 12, 2015·Avian Pathology : Journal of the W.V.P.A·Grzegorz Woźniakowski, Karolina Tarasiuk
Feb 5, 2013·Journal of Virological Methods·Qin HeXiaoyue Chen
Aug 26, 2018·Transboundary and Emerging Diseases·Mohamed El-TholothKamel I Abou El-Azm
Mar 23, 2017·The Veterinary Quarterly·Kuldeep DhamaRaj Kumar Singh

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