Rapid Differentiation of Host and Parasitic Exosome Vesicles Using Microfluidic Photonic Crystal Biosensor

ACS Sensors
Yifei WangLiang Dong

Abstract

Parasite extracellular vesicles (EVs) are potential biomarkers that could be exploited for the diagnosis of infectious disease. This paper reports a rapid bioassay to discriminate parasite and host EVs. The EV detection assay utilizes a label-free photonic crystal (PC) biosensor to detect the EVs using a host-specific transmembrane protein (CD63), which is present on EV secreted by host cells (modeled by murine macrophage cell line J774A.1) but is not expressed on EV secreted by parasitic nematodes such as the gastrointestinal nematode Ascaris suum. The surface of PC is functionalized to recognize CD63, and is sensitive to the changes in refractive index caused by the immobilization of EVs. The biosensor demonstrates a detection limit of 2.18 × 109 EVs/mL and a capability to characterize the affinity constants of antibody-host EV bindings. The discrimination of murine host EVs from parasite EVs indicates the capability of the sensor to differentiate EVs from different origins. The label-free, rapid EV assay could be used to detection parasite infection and facilitate the exosome-based clinic diagnosis and exosome research.

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Citations

Feb 20, 2020·Sensors·Jin-Ha ChoiJeong-Woo Choi
Dec 16, 2020·Journal of Extracellular Vesicles·Hannah J LoghryMichael J Kimber
Dec 24, 2020·Biosensors·Yung-Tsan ChenJian-Jang Huang
Jul 13, 2021·Frontiers in Cellular and Infection Microbiology·Yi MuPengfei Cai

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