Rapid discriminative detection of dengue viruses via loop mediated isothermal amplification

Talanta
Jong-Gil KimTae Jung Park

Abstract

Dengue virus (DENV) is one of the life-threatening viruses to the human. In this study, we have designed specific novel primers for rapid discriminative detection of DENV-1, DENV-2, and DENV-4 by real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) reaction. The effect of parameters such as reaction temperature and magnesium sulfate was investigated on the RT-LAMP reaction for detection of DENV RNA. Under the optimal conditions, this method is able to differentiate and to detect DENV within 25 min, exhibiting detection limit of 3.5 copies/μL. Importantly, the novel specific primers-based RT-LAMP assay did not react with other viruses, suggesting the selectivity of the method towards DENV RNA. The RT-LAMP reaction products are easily visualized with naked-eye when irradiated them under UV light at 365 nm. Amplification products could be visualized directly for color changes. This method provides a facile, and accurate molecular amplication technique for the rapid discriminative detection of dengue viruses. The RT-LAMP platform can be used as a promissing diagnostic tool for discriminative detection of DENV without aid of complicated protocols or sophisticated equipment.

Citations

Apr 10, 2019·Vector Borne and Zoonotic Diseases·Hao LiuXi-Jun Yan
Jul 19, 2019·Biosensors & Bioelectronics·J R AnushaC Justin Raj
Feb 20, 2021·Materials Science for Energy Technologies·Victoria Xin Ting ZhaoXiaodong Zhou
Dec 15, 2020·Journal of Hazardous Materials·Mohsen PilevarWoo Hyoung Lee
Sep 6, 2020·Talanta·Elena PinchonChantal Fournier-Wirth

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